Many proteins bind to the activated platelet derived growth factor receptor (PDGF-R) either directly or by means of adapter molecules. Up to now all these proteins were shown to transmit and amplify the signal started with PDGF-R stimulation. In a recent study our group has demonstrated that lowMrphosphotyrosine protein phosphatase (LMW-PTP) specifically interacts with PDGF-R in NIH3T3 cells. In the present study we have attempted to clarify the modality of interaction, bothin vivoandin vitro,of these two proteins, using a catalytically inactive LMW-PTP mutant. Our results indicate that LMW-PTP and PDGF-R interact directly, without the necessity of any adapter protein. This interaction leads to PDGF-R dephosphorylation and, presumably, interrupts one or more of the mitogenic pathways that originate from receptor activation.

Low M(r) phosphotyrosine protein phosphatase interacts with the PDGF receptor directly via its catalytic site / P. Chiarugi; P. Cirri; G. Raugei; G. Manao; L. Taddei; G. Ramponi. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 219:(1996), pp. 21-25. [10.1006/bbrc.1996.0174]

Low M(r) phosphotyrosine protein phosphatase interacts with the PDGF receptor directly via its catalytic site

CHIARUGI, PAOLA;CIRRI, PAOLO;RAUGEI, GIOVANNI;MANAO, GIAMPAOLO;TADDEI, MARIA LETIZIA;RAMPONI, GIAMPIETRO
1996

Abstract

Many proteins bind to the activated platelet derived growth factor receptor (PDGF-R) either directly or by means of adapter molecules. Up to now all these proteins were shown to transmit and amplify the signal started with PDGF-R stimulation. In a recent study our group has demonstrated that lowMrphosphotyrosine protein phosphatase (LMW-PTP) specifically interacts with PDGF-R in NIH3T3 cells. In the present study we have attempted to clarify the modality of interaction, bothin vivoandin vitro,of these two proteins, using a catalytically inactive LMW-PTP mutant. Our results indicate that LMW-PTP and PDGF-R interact directly, without the necessity of any adapter protein. This interaction leads to PDGF-R dephosphorylation and, presumably, interrupts one or more of the mitogenic pathways that originate from receptor activation.
1996
219
21
25
P. Chiarugi; P. Cirri; G. Raugei; G. Manao; L. Taddei; G. Ramponi
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/322481
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 31
  • ???jsp.display-item.citation.isi??? 31
social impact