Acyl phosphatase has been purified from horse liver. The purification procedure consisted of an acetic acid extraction, an isoelectric precipitation of foreign proteins and three subsequent ion-exchange chromatographies. The molecular weight was determined by sodium dodecylsulfate-polyacrylamide gel electrophoresis and by Sephadex G-75 gel filtration and the amino acid composition by ion-exchange chromatography. The above properties and the substrate specificity were compared with those found for horse muscle acyl phosphatase.
Purification and some molecular properties of horse liver acyl phosphatase / G. Ramponi; P. Nassi; G. Cappugi; C. Treves; G. Manao. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - STAMPA. - 284:(1972), pp. 485-496. [10.1016/0005-2744(72)90147-7]
Purification and some molecular properties of horse liver acyl phosphatase
RAMPONI, GIAMPIETRO;NASSI, PAOLO ANTONIO;CAPPUGI, GIANNI;TREVES, CRISTINA;MANAO, GIAMPAOLO
1972
Abstract
Acyl phosphatase has been purified from horse liver. The purification procedure consisted of an acetic acid extraction, an isoelectric precipitation of foreign proteins and three subsequent ion-exchange chromatographies. The molecular weight was determined by sodium dodecylsulfate-polyacrylamide gel electrophoresis and by Sephadex G-75 gel filtration and the amino acid composition by ion-exchange chromatography. The above properties and the substrate specificity were compared with those found for horse muscle acyl phosphatase.
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