The use of an affinity chromatography step performed with an immunoadsorbent consisting of anti-horse muscle acylphosphatase antibodies covalently linked to Sepharose 4B allowed us to purify horse heart acylphosphatase in a very rapid and efficient fashion. As in skeletal muscle, also in heart the enzyme is present as both a mixed disulfide with glutathione and a S-S dimer. The abundance of these forms in heart is quite lower than in skeletal muscle. The comparison of the molecular forms so purified with those obtained from horse skeletal muscle showed the same aminoacid composition, tryptic fingerprint, together with strictly similar apparent molecular weight and main kinetic parameters, supporting the conclusion that the acylphosphatase present in heart is the same enzyme as that purified from skeletal muscle.
Horse heart acylphosphatase: purification and characterization / M. Stefani; G. Camici; G. Manao; G. Cappugi; G. Liguri; D. Degl'Innocenti; N. Landi; A. Berti. - In: ITALIAN JOURNAL OF BIOCHEMISTRY. - ISSN 0021-2938. - STAMPA. - 34:(1985), pp. 94-108.
Horse heart acylphosphatase: purification and characterization
STEFANI, MASSIMO;CAMICI, GUIDO;MANAO, GIAMPAOLO;CAPPUGI, GIANNI;LIGURI, GIANFRANCO;DEGL'INNOCENTI, DONATELLA;BERTI, ANDREA
1985
Abstract
The use of an affinity chromatography step performed with an immunoadsorbent consisting of anti-horse muscle acylphosphatase antibodies covalently linked to Sepharose 4B allowed us to purify horse heart acylphosphatase in a very rapid and efficient fashion. As in skeletal muscle, also in heart the enzyme is present as both a mixed disulfide with glutathione and a S-S dimer. The abundance of these forms in heart is quite lower than in skeletal muscle. The comparison of the molecular forms so purified with those obtained from horse skeletal muscle showed the same aminoacid composition, tryptic fingerprint, together with strictly similar apparent molecular weight and main kinetic parameters, supporting the conclusion that the acylphosphatase present in heart is the same enzyme as that purified from skeletal muscle.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.