The level of both isoforms of acylphosphatase was evaluated in the human erythroleukemia K562 cell line during differentiation. K562 cells were treated with PMA, which induces megakaryocytic differentiation, and with aphidicolin or hemin, which stimulate erythrocytic differentiation. While the MT isoform showed an average 10-fold increase independently of the differentiating agent used, only hemin treatment caused a similar increase of the CT isoform, suggesting a different role of the two isoforms in the cell. Treatment with either hemin or aphidicolin of K562 cells overexpressing the two acylphosphatase isoforms suggested the possibility that acylphosphatases play a role in the onset of differentiation.
Acylphosphatase is involved in differentiation of K562 cells / P.Chiarugi; D.Degl'Innocenti; L.Taddei; G.Raugei; A.Berti; S.Rigacci; G.Ramponi. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - STAMPA. - 4:(1997), pp. 334-340.
Acylphosphatase is involved in differentiation of K562 cells
CHIARUGI, PAOLA;DEGL'INNOCENTI, DONATELLA;TADDEI, MARIA LETIZIA;RAUGEI, GIOVANNI;BERTI, ANDREA;RIGACCI, STEFANIA;RAMPONI, GIAMPIETRO
1997
Abstract
The level of both isoforms of acylphosphatase was evaluated in the human erythroleukemia K562 cell line during differentiation. K562 cells were treated with PMA, which induces megakaryocytic differentiation, and with aphidicolin or hemin, which stimulate erythrocytic differentiation. While the MT isoform showed an average 10-fold increase independently of the differentiating agent used, only hemin treatment caused a similar increase of the CT isoform, suggesting a different role of the two isoforms in the cell. Treatment with either hemin or aphidicolin of K562 cells overexpressing the two acylphosphatase isoforms suggested the possibility that acylphosphatases play a role in the onset of differentiation.
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