Among methods proposed for the study of the activity of metabolic systems in man, the measurement of 6β-OH-cortisol in urine has been suggested as a possible alternative to the direct measurement of the metabolism of exogenous chemicals. Until recently, however, methods for the determination of 6β-OH-cortisol in urine were cumbersome and unreliable, being based upon paper chromatography or chemical oxidation followed by gas chromatography. A method based on liquid-liquid chromatography has been recently described by Roots et al. We have independently developed an alternative liquid-liquid chromatography fractionation procedure that has some advantages over the published one, and this will be described here
A new method for the determination of 6-beta-OH-cortisol in human urine / M. Lodovici; P. Dolara; P. Bavazzano; A. Colzi; V. Pistolesi. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 114:(1981), pp. 107-110.
A new method for the determination of 6-beta-OH-cortisol in human urine.
LODOVICI, MAURA;DOLARA, PIERO;
1981
Abstract
Among methods proposed for the study of the activity of metabolic systems in man, the measurement of 6β-OH-cortisol in urine has been suggested as a possible alternative to the direct measurement of the metabolism of exogenous chemicals. Until recently, however, methods for the determination of 6β-OH-cortisol in urine were cumbersome and unreliable, being based upon paper chromatography or chemical oxidation followed by gas chromatography. A method based on liquid-liquid chromatography has been recently described by Roots et al. We have independently developed an alternative liquid-liquid chromatography fractionation procedure that has some advantages over the published one, and this will be described here
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