The BALB 3T3 clone A31-1-1 mouse embryo fibroblast cell line at passage 7-13 was used for studying neoplastic transformation with a mutagen isolated from beef extract and identified as 2-amino-3 methylimidazo [4,5-d] quinoline (IQ). IQ at a concentration of 1, 5 and 15 ng/ml induced transformed type III foci. No foci were detected in control plates treated with DMSO alone. At the highest concentration of IQ (30 ng/ml) no transformed type III foci were observed and plating efficiency was reduced. The results demonstrate a positive transforming capability of this compound on mammalian cells.
Neoplastic transformation of BALB 3T3 mouse embryo fibroblasts by the beef extract mutagen 2-amino-3-methylimidazo [4,5-d] quinoline / E. Cortesi; P. Dolara. - In: CANCER LETTERS. - ISSN 0304-3835. - STAMPA. - 20:(1983), pp. 43-47.
Neoplastic transformation of BALB 3T3 mouse embryo fibroblasts by the beef extract mutagen 2-amino-3-methylimidazo [4,5-d] quinoline.
DOLARA, PIERO
1983
Abstract
The BALB 3T3 clone A31-1-1 mouse embryo fibroblast cell line at passage 7-13 was used for studying neoplastic transformation with a mutagen isolated from beef extract and identified as 2-amino-3 methylimidazo [4,5-d] quinoline (IQ). IQ at a concentration of 1, 5 and 15 ng/ml induced transformed type III foci. No foci were detected in control plates treated with DMSO alone. At the highest concentration of IQ (30 ng/ml) no transformed type III foci were observed and plating efficiency was reduced. The results demonstrate a positive transforming capability of this compound on mammalian cells.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.