Sarcoplasmic reticulum (SR) native vesicles incorporating Ca-ATPase are adsorbed on a solid-supported lipid membrane (SSM). Upon adsorption, the ion pumps are chemically activated by concentration jumps of ATP and the capacitive current transients generated by SR Ca-ATPase are measured under potentiostatic conditions. The Michaelis-Menten constant, Km, for ATP is evaluated by varying the concentration of ATP in the activating solution. This preliminary result shows that ion transport by SR Ca-ATPase can be suitably investigated by a technique based on concentration jumps on an SSM.
Calcium transport by Sarcoplasmic Reticulum Ca-ATPase can be investigated on a solid-supported membrane / G. BARTOLOMMEI; F. TADINI-BUONINSEGNI; M.R. MONCELLI. - In: BIOELECTROCHEMISTRY. - ISSN 1567-5394. - STAMPA. - 63:(2004), pp. 157-160. [10.1016/j.bioelechem.2003.07.010]
Calcium transport by Sarcoplasmic Reticulum Ca-ATPase can be investigated on a solid-supported membrane
BARTOLOMMEI, GIANLUCA;TADINI BUONINSEGNI, FRANCESCO;MONCELLI, MARIA ROSA
2004
Abstract
Sarcoplasmic reticulum (SR) native vesicles incorporating Ca-ATPase are adsorbed on a solid-supported lipid membrane (SSM). Upon adsorption, the ion pumps are chemically activated by concentration jumps of ATP and the capacitive current transients generated by SR Ca-ATPase are measured under potentiostatic conditions. The Michaelis-Menten constant, Km, for ATP is evaluated by varying the concentration of ATP in the activating solution. This preliminary result shows that ion transport by SR Ca-ATPase can be suitably investigated by a technique based on concentration jumps on an SSM.
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