In this study we have investigated responsiveness to progesterone in spermatozoa from a group of unselected male partners of couples undergoing in-vitro fertilization (IVF). We evaluated progesterone-stimulated intracellular Ca2+ ([Ca2+](i)) and percentage increase in acrosome reaction in the same sperm sample used for oocyte inseminations, [Ca2+](i) was measured with a fluorimetric method, while the acrosome reaction was assessed using a fluorescent probe (fluorescein isothiocyanate-labelled peanut lectin), The average percentage [Ca2+](i) as well as the rate of increase in the frequency of acrosome reaction following progesterone challenge were significantly lower (P < 0.005) in the group of patients with a fertilization rate < 50%, In addition, significant correlations between the fertilization rate and the progesterone-stimulated [Ca2+](i) and acrosome reaction increases (r = 0.78 and r 0.79 respectively) were observed, Furthermore, in cases of fertilization failure, no increase of [Ca2+](i) or acrosome reaction was observed in response to progesterone with the exception of one case, Our results indicate that [Ca2+](i) and acrosome reaction increases in response to progesterone can be of value in the prediction of sperm fertilizing ability, As the two parameters were significantly correlated to each other (r = 0.86), the two assays have similar IVF predictive value and might be used interchangeably as a diagnostic tool in the assignment of male patients to the different kinds of assisted fertilization techniques.
Intracellular calcium increase and acrosome reaction in response to progesterone in human spermatozoa are correlated with in-vitro fertilization / C. Krausz; L. Bonaccorsi; M. Luconi; B. Fuzzi; L. Criscuoli; S. Pellegrini; G. Forti; E. Baldi. - In: HUMAN REPRODUCTION. - ISSN 0268-1161. - STAMPA. - 10:(1995), pp. 120-124. [10.1093/humrep/10.1.120]
Intracellular calcium increase and acrosome reaction in response to progesterone in human spermatozoa are correlated with in-vitro fertilization.
KRAUSZ, CSILLA GABRIELLA;BONACCORSI, LORELLA;LUCONI, MICHAELA;FUZZI, BEATRICE;CRISCUOLI, LUCIANA;PELLEGRINI, SANDRA;FORTI, GIANNI;BALDI, ELISABETTA
1995
Abstract
In this study we have investigated responsiveness to progesterone in spermatozoa from a group of unselected male partners of couples undergoing in-vitro fertilization (IVF). We evaluated progesterone-stimulated intracellular Ca2+ ([Ca2+](i)) and percentage increase in acrosome reaction in the same sperm sample used for oocyte inseminations, [Ca2+](i) was measured with a fluorimetric method, while the acrosome reaction was assessed using a fluorescent probe (fluorescein isothiocyanate-labelled peanut lectin), The average percentage [Ca2+](i) as well as the rate of increase in the frequency of acrosome reaction following progesterone challenge were significantly lower (P < 0.005) in the group of patients with a fertilization rate < 50%, In addition, significant correlations between the fertilization rate and the progesterone-stimulated [Ca2+](i) and acrosome reaction increases (r = 0.78 and r 0.79 respectively) were observed, Furthermore, in cases of fertilization failure, no increase of [Ca2+](i) or acrosome reaction was observed in response to progesterone with the exception of one case, Our results indicate that [Ca2+](i) and acrosome reaction increases in response to progesterone can be of value in the prediction of sperm fertilizing ability, As the two parameters were significantly correlated to each other (r = 0.86), the two assays have similar IVF predictive value and might be used interchangeably as a diagnostic tool in the assignment of male patients to the different kinds of assisted fertilization techniques.File | Dimensione | Formato | |
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