Characterization of [3H]substance P binding sites in human skin.

Bianchi, Beatrice; Matucci, Rosanna; Danesi, A.; Rossi, R.; Ipponi, P.; Giannotti, Benvenuto; Johansson, O.; Cappugi, Pietro

Radioligand binding experiments were performed with crude homogenates from normal human skin in order to investigate substance P receptor density. Binding of [3H]substance P ([3H]SP) reached equilibrium after 20 min and was saturable analysis of saturation curves gave a significantly better fit using two-site binding compared to the single-site model. Competition studies employing some selective agonists for NK1, NK2 and NK3 receptors have demonstrated that only the NK1 selective agonist, [Sar9, Met(O2)11]-SP, was a competitor for [3H]SP binding. In addition, the non-hydrolyzable guanosine 5'-0-(3-thiotriphosphate) altered the dissociation of SP from NK1 receptors by increasing the number of low-affinity sites. These data show that in the skin [3H]SP binds to a single population of substance P high-affinity sites, which represent NK1-type receptors.

trova@unifi

Titolo:	Characterization of [3H]substance P binding sites in human skin.
Autori di Ateneo:	BIANCHI, BEATRICE MATUCCI, ROSANNA A. Danesi R. Rossi P. Ipponi GIANNOTTI, BENVENUTO O. Johansson CAPPUGI, PIETRO mostra contributor esterni nascondi contributor esterni
Autori:	BIANCHI, BEATRICE; MATUCCI, ROSANNA; A. Danesi; R. Rossi; P. Ipponi; GIANNOTTI, BENVENUTO; O. Johansson; CAPPUGI, PIETRO
Data di pubblicazione:	1999
Rivista:	JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
Volume:	12
Pagina iniziale:	6
Pagina finale:	10
Abstract:	Radioligand binding experiments were performed with crude homogenates from normal human skin in order to investigate substance P receptor density. Binding of [3H]substance P ([3H]SP) reached equilibrium after 20 min and was saturable analysis of saturation curves gave a significantly better fit using two-site binding compared to the single-site model. Competition studies employing some selective agonists for NK1, NK2 and NK3 receptors have demonstrated that only the NK1 selective agonist, [Sar9, Met(O2)11]-SP, was a competitor for [3H]SP binding. In addition, the non-hydrolyzable guanosine 5'-0-(3-thiotriphosphate) altered the dissociation of SP from NK1 receptors by increasing the number of low-affinity sites. These data show that in the skin [3H]SP binds to a single population of substance P high-affinity sites, which represent NK1-type receptors.
Handle:	http://hdl.handle.net/2158/346040
Appare nelle tipologie:	1a - Articolo su rivista

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