The carbon centered radicals generated by preincubation of cumene hydroperoxide with rat cardiac membranes dose dependently reduced the Bmax of [3H]nitrendipine binding, while KD was unchanged. The reduction induced by cumene hydroperoxide was prevented by 2,6-di-t-butlyl-4-methyl-phenol. The generation of OH did not influence the [3H]nitrendipine binding; also other oxidative agents (H2O2 and HClO4) did not modify this binding. Therefore the reduction in [3H]nitrendipine binding sites is not attributable to generic oxidative stress but to the formation of carbon centered radicals.

Carbon centered radicals reduce the density of L-type calcium channels in rat cardiac membranes / R. Matucci; B. Bianchi; M.F. Ottaviani; S. Campana; F. Bennardini; F. Franconi. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 223:(1996), pp. 85-90.

Carbon centered radicals reduce the density of L-type calcium channels in rat cardiac membranes.

MATUCCI, ROSANNA;BIANCHI, BEATRICE;OTTAVIANI, MARIA FRANCESCA;FRANCONI, FLAVIA
1996

Abstract

The carbon centered radicals generated by preincubation of cumene hydroperoxide with rat cardiac membranes dose dependently reduced the Bmax of [3H]nitrendipine binding, while KD was unchanged. The reduction induced by cumene hydroperoxide was prevented by 2,6-di-t-butlyl-4-methyl-phenol. The generation of OH did not influence the [3H]nitrendipine binding; also other oxidative agents (H2O2 and HClO4) did not modify this binding. Therefore the reduction in [3H]nitrendipine binding sites is not attributable to generic oxidative stress but to the formation of carbon centered radicals.
1996
223
85
90
R. Matucci; B. Bianchi; M.F. Ottaviani; S. Campana; F. Bennardini; F. Franconi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/346047
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