FLORE (FLOrence REsearch) è il repository istituzionale ad accesso pieno e aperto dell'Università degli Studi di Firenze.
Raccoglie, documenta e conserva le informazioni sulla produzione scientifica dell'Università e costituisce l'Anagrafe della ricerca dell'Ateneo.
EnglishWe have identified an estrogen receptor of approximately 29 kDa apparent molecular weight in human sperm membranes by ligand and Western blot analysis, respectively, using peroxidase-conjugated E-2 and an antibody directed against the ligand binding region of the genomic receptor (alpha H222). Such receptor is functional since 17 beta E-2 induces a rapid and sustained increase of intracellular calcium concentrations ([Ca2+](i)) which is completely eliminated by preincubation with alpha H222. 17 beta E-2 effects on calcium are clearly mediated by a membrane receptor, as they are reproduced by the membrane-impermeable conjugate of the hormone BSA-E-2. Dose-response curve for this effect is biphasic with EC(50)s in the nanomolar and micromolar range. In addition to calcium increase, 17 beta E-2 stimulates tyrosine phosphorylation of several sperm proteins including the 29-kDa protein band. Preincubation of human sperm with 17 beta E-2 inhibits calcium and acrosome reaction increases in response to progesterone. We conclude that estrogens may play a role in the modulation of non-genomic action of progesterone (P) in human sperm during the process of fertilization. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
A novel functional estrogen receptor on human sperm membrane interferes with progesterone effects / E. BALDI; M. LUCONI; M. MURATORI; G. FORTI. - In: MOLECULAR AND CELLULAR ENDOCRINOLOGY. - ISSN 0303-7207. - STAMPA. - 161(2000), pp. 31-35. [10.1016/S0303-7207(99)00220-8]
| Titolo: | A novel functional estrogen receptor on human sperm membrane interferes with progesterone effects. | |
| Autori di Ateneo: | ||
| Autori: | BALDI, ELISABETTA; LUCONI, MICHAELA; MURATORI, MONICA; FORTI, GIANNI | |
| Anno di registrazione: | 2000 | |
| Rivista: | ||
| Volume: | 161 | |
| Pagina iniziale: | 31 | |
| Pagina finale: | 35 | |
| Abstract: | We have identified an estrogen receptor of approximately 29 kDa apparent molecular weight in human sperm membranes by ligand and Western blot analysis, respectively, using peroxidase-conjugated E-2 and an antibody directed against the ligand binding region of the genomic receptor (alpha H222). Such receptor is functional since 17 beta E-2 induces a rapid and sustained increase of intracellular calcium concentrations ([Ca2+](i)) which is completely eliminated by preincubation with alpha H222. 17 beta E-2 effects on calcium are clearly mediated by a membrane receptor, as they are reproduced by the membrane-impermeable conjugate of the hormone BSA-E-2. Dose-response curve for this effect is biphasic with EC(50)s in the nanomolar and micromolar range. In addition to calcium increase, 17 beta E-2 stimulates tyrosine phosphorylation of several sperm proteins including the 29-kDa protein band. Preincubation of human sperm with 17 beta E-2 inhibits calcium and acrosome reaction increases in response to progesterone. We conclude that estrogens may play a role in the modulation of non-genomic action of progesterone (P) in human sperm during the process of fertilization. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved. | |
| Handle: | http://hdl.handle.net/2158/348 | |
| Appare nelle tipologie: | 1a - Articolo su rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Baldi MolCellEndocrinol2000.pdf | Versione finale referata (Postprint, Accepted manuscript) | Open Access | Accesso pubblicoVisualizza/Apri |
http://hdl.handle.net/2158/348
Copyright © 2022