Optimized strategies for the synthesis of biomolecules by the microwave approach

Peptides mimicking proteins involved in disease activity are valuable synthetic tools for immunochemistry. Speediness represents a critical factor for the growing demand of such probes affecting the process of discovery of diagnostic! prognostic immunoassays. Conventional Solid-Phase Synthesis remains the principal strategy for production of immunochemical probes. Recently, the use of microwave energy was proposed to fulfil the requirement to couple a high-speed technology with an efficient solid-phase synthetic approach. Researchers are attracted by microwave-assisted SPPS that enhances coupling rates and yields by decreasing chain aggregation and possibly preventing side reactions. Therefore, microwave energy has been described to be particularly valuable for N-glycosylated amino acids, preparation of complex peptide sequences, i.e. glycopeptides and multiple antigenic peptides, and highly amphipathic peptides, as synthetic probes for specific and high affinity diagnostic immunoassays to follow up disease activity.