A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies
A strategy for in situ detection of natural transformation-based horizontal gene transfer events / Rizzi A; Pontiroli A; Brusetti L; Borin S; Sorlini C; Abruzzese A; Sacchi GA; Vogel TM; Simonet P; M.BAZZICALUPO; Nielsen KM; Monier JM; Daffonchio D. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 0099-2240. - STAMPA. - 74:(2008), pp. 1250-1254.
A strategy for in situ detection of natural transformation-based horizontal gene transfer events
BAZZICALUPO, MARCO;
2008
Abstract
A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies
| File | Dimensione | Formato | |
|---|---|---|---|
|
AEM 2008 in situ HGT.pdf
Accesso chiuso
Tipologia:
Versione finale referata (Postprint, Accepted manuscript)
Licenza:
Tutti i diritti riservati
Dimensione
408.22 kB
Formato
Adobe PDF
|
408.22 kB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
