A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies

A strategy for in situ detection of natural transformation-based horizontal gene transfer events / Rizzi A; Pontiroli A; Brusetti L; Borin S; Sorlini C; Abruzzese A; Sacchi GA; Vogel TM; Simonet P; M.BAZZICALUPO; Nielsen KM; Monier JM; Daffonchio D. - In: APPLIED AND ENVIRONMENTAL MICROBIOLOGY. - ISSN 0099-2240. - STAMPA. - 74:(2008), pp. 1250-1254.

A strategy for in situ detection of natural transformation-based horizontal gene transfer events

BAZZICALUPO, MARCO;
2008

Abstract

A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies
2008
74
1250
1254
Rizzi A; Pontiroli A; Brusetti L; Borin S; Sorlini C; Abruzzese A; Sacchi GA; Vogel TM; Simonet P; M.BAZZICALUPO; Nielsen KM; Monier JM; Daffonchio D...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/352168
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