Monitoring biological sulphide oxidation processes with combined respirometric and titrimetric techniques

The application of respirometric and titrimetric techniques to evaluate kinetic parameters and stoichiometry of the sulphide-oxidising biomass is a new promising approach for biotechnological sulphide oxidation process monitoring. It was possible to estimate the yield coefficients of each oxidation step of sulphide to elemental sulphur and to sulphate using respirometric tests, while evaluating the behaviour of the biomass in endogenous conditions. Furthermore, it was demonstrated how the combined application of titrimetric and respirometric techniques enabled the monitoring of sulphur and sulphate formation as a function of the environmental conditions. This approach provided valuable information of the biological sulphide oxidation processes and preliminary results may be used as a starting point for the formulation and use of a mathematical model.