The entrapment of AMP, GMP, CMP, and UMP nucleotides along with two different AMP-based nucleolipids (hydrophobically functionalized nucleotides) inside the liquid crystalline phases of the monoolein/water system is investigated through optical microscopy, small-angle X-ray diffraction (SAXRD), and nuclear magnetic resonance (NMR) techniques. As ascertained mainly through 31P NMR experiments, when included within the cubic phase, the various nucleotides undergo a slow hydrolysis of the sugar−phosphate ester bond, induced by specific interactions at the monoolein-water interface. Upon aging, the degradation of the nucleotides causes a cubic-to-hexagonal phase transition. Differently, neither hydrolysis nor alterations of the monoolein self-assembly are observed when the nucleotides are included as lipid derivatives within the cubic liquid crystalline phase. A model that explains both the hydrolysis and the consequent phase transition is presented

Orientation and Specific Interactions of Nucleotides and Nucleolipids Inside Monoolein-Based Liquid Crystals / S. Murgia; S. Lampis; R. Angius; D. Berti; M. Monduzzi. - In: JOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL. - ISSN 1520-6106. - STAMPA. - 113:(2009), pp. 9205-9215. [10.1021/jp9035474]

Orientation and Specific Interactions of Nucleotides and Nucleolipids Inside Monoolein-Based Liquid Crystals

BERTI, DEBORA;
2009

Abstract

The entrapment of AMP, GMP, CMP, and UMP nucleotides along with two different AMP-based nucleolipids (hydrophobically functionalized nucleotides) inside the liquid crystalline phases of the monoolein/water system is investigated through optical microscopy, small-angle X-ray diffraction (SAXRD), and nuclear magnetic resonance (NMR) techniques. As ascertained mainly through 31P NMR experiments, when included within the cubic phase, the various nucleotides undergo a slow hydrolysis of the sugar−phosphate ester bond, induced by specific interactions at the monoolein-water interface. Upon aging, the degradation of the nucleotides causes a cubic-to-hexagonal phase transition. Differently, neither hydrolysis nor alterations of the monoolein self-assembly are observed when the nucleotides are included as lipid derivatives within the cubic liquid crystalline phase. A model that explains both the hydrolysis and the consequent phase transition is presented
113
9205
9215
S. Murgia; S. Lampis; R. Angius; D. Berti; M. Monduzzi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/371304
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