The regulatory sequences of Azospirillum brasilense Sp7 nifH gene were fused with the cam reporter gene and used for studying the factors controlling nifH transcription. A DNA sequence, downstream the ATG codon of nifH, that could be involved in the negative regulation of nifH transcription, was identified. The effect of 1 and 2 mM of ammonium on the transcription of the A. brasilense nifH gene and on the nitrogenase activity, in the presence of the Klebsiella pneumoniae NifA protein, was examined.
Control of nifH transcription in Azospirillum brasilense: involvement of NifA and cis-acting sequences / S. Fancelli; R. Fani; A. Grifoni; M. Mugnai; R. Pastorelli; M. Bazzicalupo. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - STAMPA. - 115:(1994), pp. 57-62.
Control of nifH transcription in Azospirillum brasilense: involvement of NifA and cis-acting sequences
FANI, RENATO;BAZZICALUPO, MARCO
1994
Abstract
The regulatory sequences of Azospirillum brasilense Sp7 nifH gene were fused with the cam reporter gene and used for studying the factors controlling nifH transcription. A DNA sequence, downstream the ATG codon of nifH, that could be involved in the negative regulation of nifH transcription, was identified. The effect of 1 and 2 mM of ammonium on the transcription of the A. brasilense nifH gene and on the nitrogenase activity, in the presence of the Klebsiella pneumoniae NifA protein, was examined.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.