Advantages of dried blood spot include low invasiveness, ease and low cost of sample collection, transport, and storage. We used tandem mass spectrometry (LC-MS/MS) to determine phenobarbital levels on dried blood spot specimens and compared this methodology to commercially available particle enhanced turbidimetric inhibition immunoassay (PETINIA) in plasma/serum samples. The calibration curve in matrix using D5-phenobarbital as internal standard was linear in the phenobarbital concentration range of 1–100 mg/L (correlation coefficient 0.9996). The coefficients of variation in blood spots ranged 2.29–6.71% and the accuracy ranged 96.54– 103.87%. There were no significant differences between the concentrations measured using PETINA and LC-MS/MS (both had similar precision and accuracy) however, LC-MS/MS allows at least 1.5 times higher throughput of phenobarbital analysis and additionally offers ease of sample collection which is particularly important for newborns or small infants.

A new rapid micromethod for the assay of phenobarbital from dried blood spots by LC-tandem mass spectrometry / la Marca G; Malvagia S; Filippi L; Luceri F; Moneti G; Guerrini R. - In: EPILEPSIA. - ISSN 0013-9580. - ELETTRONICO. - 50(12):(2009), pp. 2658-2662.

A new rapid micromethod for the assay of phenobarbital from dried blood spots by LC-tandem mass spectrometry

LA MARCA, GIANCARLO;MONETI, GLORIANO;GUERRINI, RENZO
2009

Abstract

Advantages of dried blood spot include low invasiveness, ease and low cost of sample collection, transport, and storage. We used tandem mass spectrometry (LC-MS/MS) to determine phenobarbital levels on dried blood spot specimens and compared this methodology to commercially available particle enhanced turbidimetric inhibition immunoassay (PETINIA) in plasma/serum samples. The calibration curve in matrix using D5-phenobarbital as internal standard was linear in the phenobarbital concentration range of 1–100 mg/L (correlation coefficient 0.9996). The coefficients of variation in blood spots ranged 2.29–6.71% and the accuracy ranged 96.54– 103.87%. There were no significant differences between the concentrations measured using PETINA and LC-MS/MS (both had similar precision and accuracy) however, LC-MS/MS allows at least 1.5 times higher throughput of phenobarbital analysis and additionally offers ease of sample collection which is particularly important for newborns or small infants.
2009
50(12)
2658
2662
la Marca G; Malvagia S; Filippi L; Luceri F; Moneti G; Guerrini R
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/385855
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