On May 7th 2007 a commercial preparation of Bacillus thuringiensis subsp. kurstaki strain HD1 (Btk-HD1) was applied by aircraft in an oak forest near Florence (Italy) to control Oak processionary moth (Thaumetopoea processionea L.). The persistence of Btk-HD1 in the oak forest was assessed collecting leaf, litter and soil samples. 24h before and 24h after the treatment all the three types of samples were collected, while 36, 84 and 122 days after the Btk-HD1 application only soil samples were collected. The presence of Btk-HD1 was verified by means of morphotyping followed by a high throughput staining methodology and PCR analysis. 24h after the treatment, Btk-HD1 was isolated both from leaves and litter, however, individuals belonging to the subsp. kurstaki were never detected among the members of the indigenous B. thuringiensis population of the soil oak forest.
Persistence of Bacillus thuringiensis subsp. kurstaki after an aerial application / C.Petrucci; G.Marchi; T. Panzavolta; R.Tiberi; G. Surico; M.Filindassi; L.Ruiu. - STAMPA. - (2010), pp. 55-55. (Intervento presentato al convegno Integrated protection in Quercus spp. forests/IOBC OILB tenutosi a Tempio Pausania Italy nel October 4-8, 2010).
Persistence of Bacillus thuringiensis subsp. kurstaki after an aerial application
MARCHI, GUIDO;PANZAVOLTA, TIZIANA;TIBERI, RIZIERO;SURICO, GIUSEPPE;
2010
Abstract
On May 7th 2007 a commercial preparation of Bacillus thuringiensis subsp. kurstaki strain HD1 (Btk-HD1) was applied by aircraft in an oak forest near Florence (Italy) to control Oak processionary moth (Thaumetopoea processionea L.). The persistence of Btk-HD1 in the oak forest was assessed collecting leaf, litter and soil samples. 24h before and 24h after the treatment all the three types of samples were collected, while 36, 84 and 122 days after the Btk-HD1 application only soil samples were collected. The presence of Btk-HD1 was verified by means of morphotyping followed by a high throughput staining methodology and PCR analysis. 24h after the treatment, Btk-HD1 was isolated both from leaves and litter, however, individuals belonging to the subsp. kurstaki were never detected among the members of the indigenous B. thuringiensis population of the soil oak forest.
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