A novel method based on the fluorescence excited solely on Aloin by a H3BO3 derivatizing procedure, allowed its rapid and selective determination among the co-occurring components in a variety of complex matrices as several Aloes dried extracts and related commercial products. HPTLC LiChrospher silica gel 60 F254S, 20cm x 10cm, plates with ethyl formate: CH3OH : H2O (100 : 14.5 : 10 v/v) as the mobile phase were used. Densitometric determinations were performed in fluorescence mode, exciting wavelength 365 nm, optical filter K540 after derivatization with H3BO3 . The method was validated giving rise to a dependable and high throughput procedure well suited to routine application. Aloin was quantified in the range of 110 - 330 ng with RSD of repeatability and intermediate precision not exceeding 2.3% and accuracy inside the acceptance limits.

Selective determination of aloin in different matrices by HPTLC densitometry in fluorescence mode / S.A.Coran; G.Bartolucci;M.Bambagiotti-Alberti. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 54(2011), pp. 422-425. [10.1016/j.jpba.2010.09.019]

Selective determination of aloin in different matrices by HPTLC densitometry in fluorescence mode

CORAN, SILVIA;BARTOLUCCI, GIAN LUCA;BAMBAGIOTTI ALBERTI, MASSIMO
2011

Abstract

A novel method based on the fluorescence excited solely on Aloin by a H3BO3 derivatizing procedure, allowed its rapid and selective determination among the co-occurring components in a variety of complex matrices as several Aloes dried extracts and related commercial products. HPTLC LiChrospher silica gel 60 F254S, 20cm x 10cm, plates with ethyl formate: CH3OH : H2O (100 : 14.5 : 10 v/v) as the mobile phase were used. Densitometric determinations were performed in fluorescence mode, exciting wavelength 365 nm, optical filter K540 after derivatization with H3BO3 . The method was validated giving rise to a dependable and high throughput procedure well suited to routine application. Aloin was quantified in the range of 110 - 330 ng with RSD of repeatability and intermediate precision not exceeding 2.3% and accuracy inside the acceptance limits.
54
422
425
S.A.Coran; G.Bartolucci;M.Bambagiotti-Alberti
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2158/394237
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