Objective: To explore oocyte competence for subsequent birth. The modified natural IVF/intracytoplasmic sperm injection (ICSI) cycle was used as an experimental model by measuring levels of cytokines, chemokines, and growth factors in individual follicular fluids (FF). Design: A retrospective blinded study. Setting: European network of research, Embryo Implantation Control (EMBIC). Patient(s): Single FF from 83 women were analyzed during a modified natural IVF/ICSI cycle, and reproducibility of follicular composition was evaluated over two cycles for 15 patients. Intervention(s): Each FF sample was blindly tested to assess levels of 26 factors by bead-based immunoassays. Main Outcome Measure(s): Each mediator was evaluated as a potential biomarker of subsequent birth by multivariate regression analysis. Result(s): A combination of both FF G-CSF and IL-15 was the optimal model to predict birth (AUCROC, 0.85). Birth rates per cycle were 48.9% (16/33) if two good-prognosis criteria were present (FF G-CSF >12 pg/mL and IL-15 <7 pg/mL) and 8% (3/36) and 0% (0/14) if, respectively, one or none were present. FF G-CSF was significantly correlated over two cycles (r ¼ .71), suggesting a possible prognostic value of its documentation. Conclusion(s): Combined follicular G-CSF and IL-15 quantification appears as an efficient and noninvasive method to define oocyte competence for subsequent successful conception in modified natural IVF/ICSI cycles.

Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles / N.Lédée; R.Frydman; A.Osipova; J.Taieb; V.Gallot; L.Lombardelli; F.Logiodice; M.Petitbarat; R.Fanchin; G.Chaouat; N.Achour-Frydman; M.P. Piccinni. - In: FERTILITY AND STERILITY. - ISSN 0015-0282. - STAMPA. - 95:(2011), pp. -94--98.

Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles.

LOMBARDELLI, LETIZIA;LOGIODICE, FEDERICA;PICCINNI, MARIE-PIERRE
2011

Abstract

Objective: To explore oocyte competence for subsequent birth. The modified natural IVF/intracytoplasmic sperm injection (ICSI) cycle was used as an experimental model by measuring levels of cytokines, chemokines, and growth factors in individual follicular fluids (FF). Design: A retrospective blinded study. Setting: European network of research, Embryo Implantation Control (EMBIC). Patient(s): Single FF from 83 women were analyzed during a modified natural IVF/ICSI cycle, and reproducibility of follicular composition was evaluated over two cycles for 15 patients. Intervention(s): Each FF sample was blindly tested to assess levels of 26 factors by bead-based immunoassays. Main Outcome Measure(s): Each mediator was evaluated as a potential biomarker of subsequent birth by multivariate regression analysis. Result(s): A combination of both FF G-CSF and IL-15 was the optimal model to predict birth (AUCROC, 0.85). Birth rates per cycle were 48.9% (16/33) if two good-prognosis criteria were present (FF G-CSF >12 pg/mL and IL-15 <7 pg/mL) and 8% (3/36) and 0% (0/14) if, respectively, one or none were present. FF G-CSF was significantly correlated over two cycles (r ¼ .71), suggesting a possible prognostic value of its documentation. Conclusion(s): Combined follicular G-CSF and IL-15 quantification appears as an efficient and noninvasive method to define oocyte competence for subsequent successful conception in modified natural IVF/ICSI cycles.
95
-94
-98
N.Lédée; R.Frydman; A.Osipova; J.Taieb; V.Gallot; L.Lombardelli; F.Logiodice; M.Petitbarat; R.Fanchin; G.Chaouat; N.Achour-Frydman; M.P. Piccinni
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2158/395108
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