This study assessed the presence of P-gp in mitochondria and its role in parental drug-sensitive (P5) and in P5-derived MDR1. cells P1(0.5) hepatocellular carcinoma (HCC) cell lines and in drug-sensitive (PSI-2) and mdr1-transfected (PN1A) NIH/3T3 cells. By using Western blot analysis, confocal laser microscopy, measurements of Rhodamine 123 transport across mitochondrial membranes, MDR1 small interfering RNA and flow cytometry analysis, experiments indicate that P-gp is expressed in mitochondria of P1(0.5) and PN1A cells and it is functionally active. Rho 123 accumulation was largely reduced in mitochondria of P1(0.5) cells as compared to those of P5 cells; the reduced uptake of fluorescence in mitochondria of MDR cells was due to P-gp-mediated Rho 123 efflux.

P-gp localization in mitochondria and its functional characterization in multiple drug-resistant cell lines / M. Solazzo ;O. FANTAPPIE';N. Lasagna ;C. Sassoli ; D. Nosi ;R. Mazzanti.. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - STAMPA. - 312:(2006), pp. 4070-4078.

P-gp localization in mitochondria and its functional characterization in multiple drug-resistant cell lines.

SOLAZZO, MICHELA;FANTAPPIE', ORNELLA;LASAGNA, NADIA;SASSOLI, CHIARA;NOSI, DANIELE;MAZZANTI, ROBERTO
2006

Abstract

This study assessed the presence of P-gp in mitochondria and its role in parental drug-sensitive (P5) and in P5-derived MDR1. cells P1(0.5) hepatocellular carcinoma (HCC) cell lines and in drug-sensitive (PSI-2) and mdr1-transfected (PN1A) NIH/3T3 cells. By using Western blot analysis, confocal laser microscopy, measurements of Rhodamine 123 transport across mitochondrial membranes, MDR1 small interfering RNA and flow cytometry analysis, experiments indicate that P-gp is expressed in mitochondria of P1(0.5) and PN1A cells and it is functionally active. Rho 123 accumulation was largely reduced in mitochondria of P1(0.5) cells as compared to those of P5 cells; the reduced uptake of fluorescence in mitochondria of MDR cells was due to P-gp-mediated Rho 123 efflux.
312
4070
4078
M. Solazzo ;O. FANTAPPIE';N. Lasagna ;C. Sassoli ; D. Nosi ;R. Mazzanti.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2158/395895
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