We performed a proteomic study to understand how Saccharomyces cerevisiae adapts its metabolism during the exponential growth on three different concentrations of glucose; this information will be necessary to understand yeast carbon metabolism in different environments. We induced a natural diauxic shift by growing yeast cells in glucose restriction thus having a fast and complete glucose exhaustion. We noticed differential expressions of groups of proteins. Cells in high glucose have a decreased growth rate during the initial phase of fermentation; in glucose restriction and in high glucose we found an over-expression of a protein (Peroxiredoxin) involved in protection against oxidative stress insult. The information obtained in our study validates the application of a proteomic approach for the identification of the molecular bases of environmental variations such as fermentation in high glucose and during a naturally induced diauxic shift.

Effect of different glucose concentrations on proteome of Saccharomyces cerevisiae / F.Guidi; F. Magherini; T. Gamberi; M. Borro; M. Simmaco; A. Modesti. - In: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS. - ISSN 1570-9639. - ELETTRONICO. - 1804:(2011), pp. 1516-1525. [10.1016/j.bbapap.2010.12.013]

Effect of different glucose concentrations on proteome of Saccharomyces cerevisiae

GUIDI, FRANCESCA;MAGHERINI, FRANCESCA;GAMBERI, TANIA;MODESTI, ALESSANDRA
2011

Abstract

We performed a proteomic study to understand how Saccharomyces cerevisiae adapts its metabolism during the exponential growth on three different concentrations of glucose; this information will be necessary to understand yeast carbon metabolism in different environments. We induced a natural diauxic shift by growing yeast cells in glucose restriction thus having a fast and complete glucose exhaustion. We noticed differential expressions of groups of proteins. Cells in high glucose have a decreased growth rate during the initial phase of fermentation; in glucose restriction and in high glucose we found an over-expression of a protein (Peroxiredoxin) involved in protection against oxidative stress insult. The information obtained in our study validates the application of a proteomic approach for the identification of the molecular bases of environmental variations such as fermentation in high glucose and during a naturally induced diauxic shift.
2011
1804
1516
1525
F.Guidi; F. Magherini; T. Gamberi; M. Borro; M. Simmaco; A. Modesti
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/397194
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