Abstract Currently, different approaches are used to select oocytes for in vitro fertilization (IVF) procedures, but they do not assure a significant association with the pregnancy outcome. Since several studies have proposed the expression of HLA-G antigens in early embryos to be a possible marker of elevated implantation rate, we have investigated the presence of soluble HLA-G molecules in 50 follicular fluids (FFs). The results have shown soluble HLA-G antigens (sHLA-G) in 19/50 (38%) FFs. Furthermore, we have related the presence of sHLA-G molecules in FFs to detection of the soluble antigens in culture supernatants of the corresponding fertilized oocyte, evidencing a significant relationship (p = 1.3×10−6; Fisher exact p-test). Specific ELISA andWestern blot approaches identified both HLA-G5 and soluble HLA-G1 molecules in FFs while immunocytochemical analysis indicated polymorphonuclear-like and granulosa cells as responsible for production of sHLA-G1 and HLA-G5 molecules. In contrast, only sHLA-G1 antigens were detected in culture supernatants of fertilized oocytes. Overall, these results suggest a role for sHLA-G molecules in the ovulatory process and propose the FFs analysis for sHLA-G molecule presence as a useful tool for oocyte selection in IVF.

Soluble HLA-G molecules in follicular fluid: a tool for oocyte selection in IVF? / Rizzo R; Fuzzi B; Stignani M; Criscuoli L; Melchiorri L; Dabizzi S; Campioni D; Lanza F; Marzola A; Branconi F; Noci I; Baricordi OR.. - In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY. - ISSN 0165-0378. - STAMPA. - 74:(2007), pp. 133-142.

Soluble HLA-G molecules in follicular fluid: a tool for oocyte selection in IVF?

FUZZI, BEATRICE;CRISCUOLI, LUCIANA;BRANCONI, FRANCESCO;NOCI, IVO;
2007

Abstract

Abstract Currently, different approaches are used to select oocytes for in vitro fertilization (IVF) procedures, but they do not assure a significant association with the pregnancy outcome. Since several studies have proposed the expression of HLA-G antigens in early embryos to be a possible marker of elevated implantation rate, we have investigated the presence of soluble HLA-G molecules in 50 follicular fluids (FFs). The results have shown soluble HLA-G antigens (sHLA-G) in 19/50 (38%) FFs. Furthermore, we have related the presence of sHLA-G molecules in FFs to detection of the soluble antigens in culture supernatants of the corresponding fertilized oocyte, evidencing a significant relationship (p = 1.3×10−6; Fisher exact p-test). Specific ELISA andWestern blot approaches identified both HLA-G5 and soluble HLA-G1 molecules in FFs while immunocytochemical analysis indicated polymorphonuclear-like and granulosa cells as responsible for production of sHLA-G1 and HLA-G5 molecules. In contrast, only sHLA-G1 antigens were detected in culture supernatants of fertilized oocytes. Overall, these results suggest a role for sHLA-G molecules in the ovulatory process and propose the FFs analysis for sHLA-G molecule presence as a useful tool for oocyte selection in IVF.
2007
74
133
142
Rizzo R; Fuzzi B; Stignani M; Criscuoli L; Melchiorri L; Dabizzi S; Campioni D; Lanza F; Marzola A; Branconi F; Noci I; Baricordi OR.
File in questo prodotto:
File Dimensione Formato  
SOLUBLE HLA G.pdf

Accesso chiuso

Tipologia: Versione finale referata (Postprint, Accepted manuscript)
Licenza: DRM non definito
Dimensione 639.09 kB
Formato Adobe PDF
639.09 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/401973
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact