Abstract: A high-affinity muscarinic receptor is detectable both in normal 3T3 mouse fibroblasts and in their transformed counterpart obtained by transfection with the oncogene EJ/T24-H-ras. However, only the transformed cell line is responsive to muscarinic agonist carbamylcholine in terms of Ca2+ influx and polyphosphoinositide hydrolysis, whereas the normal cell line is unresponsive. Using a point-mutated p21ras protein and monoclonal antibodies anti-p21ras, we provide evidences that p21ras couples to receptor-operating calcium channels and to polyphosphoinositide hydrolysis a muscarinic receptor which is uncoupled in normal mouse fibroblasts.
Point-mutated p2lras couples a muscarinic receptor to calcium channels and polyphosphoinositide hydrolysis / V. CHIARUGI; F. PASQUALI; S. VANNUCCHI; M. RUGGIERO. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 141:(1986), pp. 591-599.
Point-mutated p2lras couples a muscarinic receptor to calcium channels and polyphosphoinositide hydrolysis
CHIARUGI, VINCENZO;VANNUCCHI, SIMONETTA;RUGGIERO, MARCO
1986
Abstract
Abstract: A high-affinity muscarinic receptor is detectable both in normal 3T3 mouse fibroblasts and in their transformed counterpart obtained by transfection with the oncogene EJ/T24-H-ras. However, only the transformed cell line is responsive to muscarinic agonist carbamylcholine in terms of Ca2+ influx and polyphosphoinositide hydrolysis, whereas the normal cell line is unresponsive. Using a point-mutated p21ras protein and monoclonal antibodies anti-p21ras, we provide evidences that p21ras couples to receptor-operating calcium channels and to polyphosphoinositide hydrolysis a muscarinic receptor which is uncoupled in normal mouse fibroblasts.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.