Exclusively heteronuclear 13C direct-detection 2D NMR experiments are performed on 13C,15N-enriched proteins in E. coli cells. Unfolded proteins or protein fragments provide well-resolved carbonyl detection, whereas folded proteins or structured motifs do not give any detectable spectrum.
13C Direct-Detection Biomolecular NMR Spectroscopy in Living Cells / I.Bertini; I.C.Felli; L.Gonnelli; M.V.Kumar; R.Pierattelli. - In: ANGEWANDTE CHEMIE. INTERNATIONAL EDITION. - ISSN 1433-7851. - STAMPA. - 50:(2011), pp. 2339-2341. [10.1002/anie.201006636]
13C Direct-Detection Biomolecular NMR Spectroscopy in Living Cells
BERTINI, IVANO;FELLI, ISABELLA CATERINA;GONNELLI, LEONARDO;MACHOHALLY VENKATESHAIAH, VASANTHA KUMAR;PIERATTELLI, ROBERTA
2011
Abstract
Exclusively heteronuclear 13C direct-detection 2D NMR experiments are performed on 13C,15N-enriched proteins in E. coli cells. Unfolded proteins or protein fragments provide well-resolved carbonyl detection, whereas folded proteins or structured motifs do not give any detectable spectrum.
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