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|Titolo:||SYNTHESIS OF LIPOPEPTIDES OF THE IMMUNODOMINANT EPITOPE HMBP(83-99) CONTAINING AMIDE OR C-C BOND LINKED HYDROPHOBIC CHAINS FOR THE STUDY OF T CELL RESPONSE|
|Autori interni:||PAPINI, ANNA MARIA|
|Data di pubblicazione:||1999|
|Rivista:||LETTERS IN PEPTIDE SCIENCE|
|Abstract:||We previously demonstrated that the lipopeptide of the myelin basic protein (MBP) immunodominant epitope in Lewis rat Palm-GpMBP(74-85) (Gp: guinea pig), which induced experimental autoimmune encephalomyelitisin vivo strongly increased the T cell proliferative responsein vitro. We extended this study to the human immunodominant epitope hMBP(83-99), synthesizing different lipophilic peptides bearing a hydrophobic chain linked through an amide or a C-C bond. To this aim, we developed a synthetic pathway for (±)-N-Fmoc-Ahd-OH (Ahd: 2-aminohexadecanoic acid) which was used to synthesize diastereomeric peptides which were successfully separated by reverse-phase high-performance liquid chromatography. MBP-specific T cell lines recognizing the immunodominant epitope hMBP(83-99) have been generated from patients affected by multiple sclerosis. Their proliferative response to the native peptide and to some lipoderivatives has been investigated. In contrast to the animal model, none of the investigated lipopeptides exhibited ‘superagonist’ activity. • Prof. L. Amaducci passed away on 11 January 1998. His memory will hearten those pursuing this research.|
|Appare nelle tipologie:||1a - Articolo su rivista|
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