Abstract: CD8+CD25+ cells, which expressed high levels of Foxp3, glucocorticoid-induced tumor necrosis factor receptor (GITR), CCR8, tumor necrosis factor receptor 2 (TNFR2), and cytotoxic T lymphocyte–associated antigen 4 (CTLA-4) mRNAs, were identified in the fibrous septa and medullary areas of human thymus. Activated CD8+CD25+ thymocytes did not produce cytokines, but most of them expressed surface CTLA-4 and transforming growth factor beta1 (TGF-beta1). Like CD4+CD25+, CD8+CD25+ thymocytes suppressed the proliferation of autologous CD25-T cells via a contact-dependent mechanism. The suppressive activity of CD8+CD25+ thymocytes was abrogated by a mixture of anti–CTLA-4 and anti–TGF-beta1 antibodies and it was mediated by their ability to inhibit the expression of the interleukin 2 receptor alpha chain on target T cells. These results demonstrate the existence of a subset of human CD8+CD25+ thymocytes sharing phenotype, functional features, and mechanism of action with CD4+CD25+ T regulatory cells. (Blood. 2003;102:4107-4114)
HUMAN CD8+CD25+ THYMOCYTES SHARE PHENOTYPIC AND FUNCTIONAL FEATURES WITH CD4+CD25+ REGULATORY THYMOCYTES / L. COSMI; F. LIOTTA; E. LAZZERI; M. FRANCALANCI; R. ANGELI; B. MAZZINGHI; V. SANTARLASCI; R. MANETTI; V. VANINI; P. ROMAGNANI; E. MAGGI; S. ROMAGNANI; F. ANNUNZIATO. - In: BLOOD. - ISSN 0006-4971. - STAMPA. - 102:(2003), pp. 4107-4114. [10.1182/blood-2003-04-1320]
HUMAN CD8+CD25+ THYMOCYTES SHARE PHENOTYPIC AND FUNCTIONAL FEATURES WITH CD4+CD25+ REGULATORY THYMOCYTES.
COSMI, LORENZO;LIOTTA, FRANCESCO;LAZZERI, ELENA;ANGELI, ROBERTA;MAZZINGHI, BENEDETTA;SANTARLASCI, VERONICA;ROMAGNANI, PAOLA;MAGGI, ENRICO;ROMAGNANI, SERGIO;ANNUNZIATO, FRANCESCO
2003
Abstract
Abstract: CD8+CD25+ cells, which expressed high levels of Foxp3, glucocorticoid-induced tumor necrosis factor receptor (GITR), CCR8, tumor necrosis factor receptor 2 (TNFR2), and cytotoxic T lymphocyte–associated antigen 4 (CTLA-4) mRNAs, were identified in the fibrous septa and medullary areas of human thymus. Activated CD8+CD25+ thymocytes did not produce cytokines, but most of them expressed surface CTLA-4 and transforming growth factor beta1 (TGF-beta1). Like CD4+CD25+, CD8+CD25+ thymocytes suppressed the proliferation of autologous CD25-T cells via a contact-dependent mechanism. The suppressive activity of CD8+CD25+ thymocytes was abrogated by a mixture of anti–CTLA-4 and anti–TGF-beta1 antibodies and it was mediated by their ability to inhibit the expression of the interleukin 2 receptor alpha chain on target T cells. These results demonstrate the existence of a subset of human CD8+CD25+ thymocytes sharing phenotype, functional features, and mechanism of action with CD4+CD25+ T regulatory cells. (Blood. 2003;102:4107-4114)File | Dimensione | Formato | |
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