Surface Plasmon Resonance Imaging for Affinity-Based Biosensors

In the last 2 decades, Surface Plasmon Resonance (SPR) sensing has played a relevant role in many biochemical and biotechnological fields, and various kinds of biorecognition elements are currently employed in a wide range of fields such as food processing, environmental monitoring and clinical/diagnostics analyses. At present, SPR-based sensing represents one of the main optical biosensor technologies. The most attractive and powerful advancement of SPR-based optical detection is the SPR imaging (SPRi) technique (also termed "SPR microscopy"), which couples the sensitivity of scanning angle SPR measurements with the spatial capabilities of imaging. In recent years SPRi has gained attention mainly in affinity-biosensors research field and actually represents a promising sensing platform for the probing of biomolecules in array format, showing to be a highly versatile system. In this work we will report about the development of a multiarray SPRi affinity sensor. In particular the system has been studied using antibody-antigen interactions food and/or clinical controls. The analytical parameters of the systems will be discussed. © 2010 Springer Science+Business Media B.V.