The oxidative folding mechanism in the intermembrane space of human mitochondria underpins a disulfide relay system consisting of the import receptor Mia40 and the homodimeric FAD-dependent thiol oxidase ALR. The flavoprotein ALR receives two electrons per subunit from Mia40, which are then donated through one-electron reactions to two cytochrome c molecules, thus mediating a switch from two-electron to one-electron transfer. We dissect here the mechanism of the electron flux within ALR, characterizing at the atomic level the ALR intermediates that allow electrons to rapidly flow to cytochrome c. The intermediate critical for the electron-transfer process implies the formation of a specific inter-subunit disulfide which exclusively allows electron flow from Mia40 to FAD. This finding allows us to present a complete model for the electron-transfer pathway in ALR

An electron-transfer path through an extended disulfide relay system: the case of the redox protein ALR / L.Banci; I.Bertini; V.Calderone; C.Cefaro; S.Ciofi-Baffoni; A.Galllo; K.Tokatlidis. - In: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. - ISSN 0002-7863. - STAMPA. - 134:(2012), pp. 1442-1445. [10.1021/ja209881f]

An electron-transfer path through an extended disulfide relay system: the case of the redox protein ALR

BANCI, LUCIA;BERTINI, IVANO;CALDERONE, VITO;CEFARO, CHIARA;CIOFI BAFFONI, SIMONE;GALLO, ANGELO;
2012

Abstract

The oxidative folding mechanism in the intermembrane space of human mitochondria underpins a disulfide relay system consisting of the import receptor Mia40 and the homodimeric FAD-dependent thiol oxidase ALR. The flavoprotein ALR receives two electrons per subunit from Mia40, which are then donated through one-electron reactions to two cytochrome c molecules, thus mediating a switch from two-electron to one-electron transfer. We dissect here the mechanism of the electron flux within ALR, characterizing at the atomic level the ALR intermediates that allow electrons to rapidly flow to cytochrome c. The intermediate critical for the electron-transfer process implies the formation of a specific inter-subunit disulfide which exclusively allows electron flow from Mia40 to FAD. This finding allows us to present a complete model for the electron-transfer pathway in ALR
2012
134
1442
1445
L.Banci; I.Bertini; V.Calderone; C.Cefaro; S.Ciofi-Baffoni; A.Galllo; K.Tokatlidis
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/606418
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