G-quadruplex structures can be formed at the single-stranded overhang of telomeric DNA, and ligands able to stabilize this structure have recently been identified as potential anticancer drugs. Among the potential G-quadruplex binders, we have studied the binding ability of berberine and sanguinarine, two members of the alkaloids family, an important class of natural products long known for medicinal purpose. Our spectroscopic (CD, NMR and fluorescence) studies and molecular modeling approaches revealed binding modes at ligand-complex stoichiometries > 1:1 and ligand self-association induced by DNA for the interactions of the natural alkaloids berberine and sanguinarine with the human telomeric G-quadruplex DNA.

Spectroscopic, molecular modeling and NMR-spectroscopic investigation of the binding mode of the natural alkaloids berberine and sanguinarine to human telomeric G-quadruplex DNA / I. Bessi; C. Bazzicalupi; C. Richter; H.R.A. Jonker; K. Saxena; C. Sissi; M. Chioccioli; S. Bianco; A.R. Bilia; H. Schwalbe; P. Gratteri. - In: ACS CHEMICAL BIOLOGY. - ISSN 1554-8929. - STAMPA. - 7:(2012), pp. 1109-1119. [10.1021/cb300096g]

Spectroscopic, molecular modeling and NMR-spectroscopic investigation of the binding mode of the natural alkaloids berberine and sanguinarine to human telomeric G-quadruplex DNA

BESSI, IRENE;BAZZICALUPI, CARLA;CHIOCCIOLI, MATTEO;BILIA, ANNA RITA;GRATTERI, PAOLA
2012

Abstract

G-quadruplex structures can be formed at the single-stranded overhang of telomeric DNA, and ligands able to stabilize this structure have recently been identified as potential anticancer drugs. Among the potential G-quadruplex binders, we have studied the binding ability of berberine and sanguinarine, two members of the alkaloids family, an important class of natural products long known for medicinal purpose. Our spectroscopic (CD, NMR and fluorescence) studies and molecular modeling approaches revealed binding modes at ligand-complex stoichiometries > 1:1 and ligand self-association induced by DNA for the interactions of the natural alkaloids berberine and sanguinarine with the human telomeric G-quadruplex DNA.
2012
7
1109
1119
I. Bessi; C. Bazzicalupi; C. Richter; H.R.A. Jonker; K. Saxena; C. Sissi; M. Chioccioli; S. Bianco; A.R. Bilia; H. Schwalbe; P. Gratteri
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/622585
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