G-quadruplex structures can be formed at the single-stranded overhang of telomeric DNA, and ligands able to stabilize this structure have recently been identified as potential anticancer drugs. Among the potential G-quadruplex binders, we have studied the binding ability of berberine and sanguinarine, two members of the alkaloids family, an important class of natural products long known for medicinal purpose. Our spectroscopic (CD, NMR and fluorescence) studies and molecular modeling approaches revealed binding modes at ligand-complex stoichiometries > 1:1 and ligand self-association induced by DNA for the interactions of the natural alkaloids berberine and sanguinarine with the human telomeric G-quadruplex DNA.
Spectroscopic, molecular modeling and NMR-spectroscopic investigation of the binding mode of the natural alkaloids berberine and sanguinarine to human telomeric G-quadruplex DNA / I. Bessi; C. Bazzicalupi; C. Richter; H.R.A. Jonker; K. Saxena; C. Sissi; M. Chioccioli; S. Bianco; A.R. Bilia; H. Schwalbe; P. Gratteri. - In: ACS CHEMICAL BIOLOGY. - ISSN 1554-8929. - STAMPA. - 7:(2012), pp. 1109-1119. [10.1021/cb300096g]
Spectroscopic, molecular modeling and NMR-spectroscopic investigation of the binding mode of the natural alkaloids berberine and sanguinarine to human telomeric G-quadruplex DNA
BESSI, IRENE;BAZZICALUPI, CARLA;CHIOCCIOLI, MATTEO;BILIA, ANNA RITA;GRATTERI, PAOLA
2012
Abstract
G-quadruplex structures can be formed at the single-stranded overhang of telomeric DNA, and ligands able to stabilize this structure have recently been identified as potential anticancer drugs. Among the potential G-quadruplex binders, we have studied the binding ability of berberine and sanguinarine, two members of the alkaloids family, an important class of natural products long known for medicinal purpose. Our spectroscopic (CD, NMR and fluorescence) studies and molecular modeling approaches revealed binding modes at ligand-complex stoichiometries > 1:1 and ligand self-association induced by DNA for the interactions of the natural alkaloids berberine and sanguinarine with the human telomeric G-quadruplex DNA.File | Dimensione | Formato | |
---|---|---|---|
Bessi_ACSChemBiol2012_1109.pdf
Accesso chiuso
Tipologia:
Versione finale referata (Postprint, Accepted manuscript)
Licenza:
Tutti i diritti riservati
Dimensione
3.77 MB
Formato
Adobe PDF
|
3.77 MB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.