Cupriavidus metallidurans CH34 is a completely sequenced soil-borne beta-proteobacterium with known genome and proteome. Comparative 2-D electrophoresis and protein mass spectrometry were used to compare the proteome of C. metallidurans CH34 from liquid culture and after incubation for 1, 3, and 12 days in microcosms containing quartz sand, kaolinite, montmorillonite, or an artificial soil. Results showed that proteome from liquid culture was similar to CH34 proteins extracted from sand and kaolinite, whereas the proteins extracted from artificial soil differed significantly and no proteins were detected from C. metallidurans CH34 incubated in the montmorillonite microcosms. Protein recovery decreased on prolonging incubation time in all microcosms. Mass spectrometry identification showed that the trend of lower recovery upon incubation time was independent on the putative function of protein. These results suggest that the soil solid phase influences the protein recovery and soil proteomic analysis and that distinction between protein recovery and protein expression in soil will be a challenging for soil proteomic researchers.

Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34 / L. Giagnoni; F. Magherini; L. Landi; S. Taghavi; D. van der Lelie; M. Puglia; L. Bianchi; L. Bini; P. Nannipieri; G. Renella; A. Modesti. - In: BIOLOGY AND FERTILITY OF SOILS. - ISSN 0178-2762. - STAMPA. - 48:(2012), pp. 425-433. [10.1007/s00374-011-0641-6]

Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34

GIAGNONI, LAURA;MAGHERINI, FRANCESCA;LANDI, LORETTA;NANNIPIERI, PAOLO;RENELLA, GIANCARLO;MODESTI, ALESSANDRA
2012

Abstract

Cupriavidus metallidurans CH34 is a completely sequenced soil-borne beta-proteobacterium with known genome and proteome. Comparative 2-D electrophoresis and protein mass spectrometry were used to compare the proteome of C. metallidurans CH34 from liquid culture and after incubation for 1, 3, and 12 days in microcosms containing quartz sand, kaolinite, montmorillonite, or an artificial soil. Results showed that proteome from liquid culture was similar to CH34 proteins extracted from sand and kaolinite, whereas the proteins extracted from artificial soil differed significantly and no proteins were detected from C. metallidurans CH34 incubated in the montmorillonite microcosms. Protein recovery decreased on prolonging incubation time in all microcosms. Mass spectrometry identification showed that the trend of lower recovery upon incubation time was independent on the putative function of protein. These results suggest that the soil solid phase influences the protein recovery and soil proteomic analysis and that distinction between protein recovery and protein expression in soil will be a challenging for soil proteomic researchers.
2012
48
425
433
L. Giagnoni; F. Magherini; L. Landi; S. Taghavi; D. van der Lelie; M. Puglia; L. Bianchi; L. Bini; P. Nannipieri; G. Renella; A. Modesti
File in questo prodotto:
File Dimensione Formato  
Soil solid phases effects on the proteomic analysis of C metallidurans CH34.pdf

Accesso chiuso

Descrizione: Soil solid phases effects on the proteomic analysis of C metallidurans CH34
Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 485.99 kB
Formato Adobe PDF
485.99 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/627008
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 12
social impact