Cerato-platanin (CP) is a protein produced by Ceratocystis platani, the causal agent of canker stain disease of plane trees. CP is the first member of the ‘cerato-platanin family’, and its role as a pathogen-associated molecular pattern (PAMP), inducing defence responses both in host and nonhost plants, is established. However, the primary role of CP and its homologues in the fungal life remains unknown. In the present work, we investigated the regulation of the cp gene during the in vitro growth of C. platani in different conditions and under the effect of potential stress factors. Fungal growth and conidiogenesis were also analysed. Results showed that cp is a single-copy gene whose expression level is strictly associated with hyphal growth and with chlamydospores formation. The analysis of a 1368 bp 5’-flanking region revealed putative motifs that could be involved in the regulation of gene expression in response to stress and developmental cues. Taking into account the localization of CP in the fungal cell wall and the recently published 3D structure of the protein, our results support a role for CP in growth and developmental processes of C. platani.

The expression of the cerato-platanin gene is related to hyphal growth and chlamydospores formation in Ceratocystis platani / I. Baccelli; C. Comparini; P.P. Bettini; F. Martellini; M. Ruocco; L. Pazzagli; R. Bernardi; A. Scala. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - STAMPA. - 327:(2012), pp. 155-163. [10.1111/j.1574-6968.2011.02475.x]

The expression of the cerato-platanin gene is related to hyphal growth and chlamydospores formation in Ceratocystis platani.

BACCELLI, IVAN;COMPARINI, CECILIA;BETTINI, PRISCILLA PAOLA;MARTELLINI, FEDERICA;PAZZAGLI, LUIGIA;SCALA, ANIELLO
2012

Abstract

Cerato-platanin (CP) is a protein produced by Ceratocystis platani, the causal agent of canker stain disease of plane trees. CP is the first member of the ‘cerato-platanin family’, and its role as a pathogen-associated molecular pattern (PAMP), inducing defence responses both in host and nonhost plants, is established. However, the primary role of CP and its homologues in the fungal life remains unknown. In the present work, we investigated the regulation of the cp gene during the in vitro growth of C. platani in different conditions and under the effect of potential stress factors. Fungal growth and conidiogenesis were also analysed. Results showed that cp is a single-copy gene whose expression level is strictly associated with hyphal growth and with chlamydospores formation. The analysis of a 1368 bp 5’-flanking region revealed putative motifs that could be involved in the regulation of gene expression in response to stress and developmental cues. Taking into account the localization of CP in the fungal cell wall and the recently published 3D structure of the protein, our results support a role for CP in growth and developmental processes of C. platani.
2012
327
155
163
I. Baccelli; C. Comparini; P.P. Bettini; F. Martellini; M. Ruocco; L. Pazzagli; R. Bernardi; A. Scala
File in questo prodotto:
File Dimensione Formato  
155.full.pdf

accesso aperto

Descrizione: Articolo principale
Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 520.61 kB
Formato Adobe PDF
520.61 kB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/645742
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 32
  • ???jsp.display-item.citation.isi??? 31
social impact