Apoptosis induction in 32D cells by IL-3 withdrawal id preceded by a drop in the intracellular calcium level

Biochem Biophys Res Commun. 1993 Aug 16;194(3):1394-7. Apoptosis induction in 32D cells by IL-3 withdrawal is preceded by a drop in the intracellular calcium level. Magnelli L, Cinelli M, Turchetti A, Chiarugi VP. Source Laboratory of Molecular Biology, Institute of General Pathology, Firenze, Italy. Abstract The 32D murine myeloid cell line is dependent on interleukin-3 (IL-3) for growth in vitro. We show here that IL-3 induces a slow increase in endocellular calcium, which is sizeable two hours after the addition. Withdrawal of the cytokine induces apoptosis, whose classic late events are evident after 16/18 hours, and are preceded by a calcium drop during the first 2/3 hours after IL-3 subtraction. Calcium drop is here proposed as a trigger of the apoptotic process, in agreement with other recently reported findings. PMID: 8352798 [PubMed - indexed for MEDLINE]