Factors affecting the growth rates of ammonium and nitrite oxidizing bacteria

The maximum specific growth rates of both ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) were investigated under varying aerobic solids retention time (SRTa) and in the presence/ absence of anoxic (alternating) conditions. Two bench SBRs, reactor R1 and R2, were run in parallel for 150 d. Reactor R1 was operated in aerobic conditions while R2 operated in alternating anoxic/aerobic conditions. The feed (synthetic wastewater), temperature, hydraulic retention time and mixing were identical in both reactors. The SRTa in both reactors was, sequentially, set at four values: 5, 4, 3 and 2 d. Kinetic tests with the biomasses from both reactors were carried out to estimate the maximum specific growth rates (lmax) at each tested SRTa and decay rates, in both aerobic and anoxic conditions. The kinetic parameters of nitrifier were estimated through the calibration of a two step nitrification–denitrification activated sludge model.