Bleomycin (blm) solutions containing the nickel(II) ion have been investigated through 1H nmr, ligand field and circular dichroism spectroscopies. It has been found the blm binds the metal ion in a pH dependent fashion. The spectral data are consistent with the presence of at least two species. It is suggested that in the low pH region blm binds to nickel(II) through the β-aminoalanino residue, whereas in the high pH region, the 4-amino-pyrimidine, imidazole, and amido group of β-hydroxy-histidine are also involved in coordination.
Nickel(II)—bleomycin: spectral investigation of the metal binding site / A Dei; D Gatteschi. - In: JOURNAL OF INORGANIC BIOCHEMISTRY. - ISSN 0162-0134. - STAMPA. - 17:(1982), pp. 131-137. [10.1016/S0162-0134(00)80081-1]
Nickel(II)—bleomycin: spectral investigation of the metal binding site
DEI, ANDREA;GATTESCHI, DANTE
1982
Abstract
Bleomycin (blm) solutions containing the nickel(II) ion have been investigated through 1H nmr, ligand field and circular dichroism spectroscopies. It has been found the blm binds the metal ion in a pH dependent fashion. The spectral data are consistent with the presence of at least two species. It is suggested that in the low pH region blm binds to nickel(II) through the β-aminoalanino residue, whereas in the high pH region, the 4-amino-pyrimidine, imidazole, and amido group of β-hydroxy-histidine are also involved in coordination.
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