With the aim of analysing the presence of sequence and/or structural polymorphisms in regulatory regions and their relationship with gene expression, promoters of the PR protein-coding genes osmotin (GenBank accession AF093743), PR1a2 (Y08844) and endochitinase (A32906) were isolated in thirteen tomato cultivars and four wild species of the genus Lycopersicon (L. pennelli, L. minutum, L. pimpinellifolium, L. peruvianum). The sequences obtained have been aligned with the ClustalW algorithm (http://www.ebi.ac.uk/clustalw/), and conserved motifs regulating gene expression in the frame of the pathogen defense response have been detected using the PLACE (http://www.dna.affrc.go.jp/PLACE/) and PlantCARE (http://sphinx.rug.ac.be:8080/PlantCARE/) databases. Finally, an analysis of the curvature has been carried out at the bend it server (http://www3.icgeb.trieste.it/~dna/ bend_it.html). The results showed that for the osmotin and PR1a2 promoters nucleotide diversity is 15-fold higher between the Lycopersicon species than between the tomato cultivars, the difference being only 2-fold for endochitinase; the latter had also the lowest inter-specific and the highest intra-specific nucleotide diversity values. Correlations beween SNPs and structural polymorphisms were found for the osmotin and PR1a2 promoters in some regulatory boxes such as the W box, GT-1 consensus, Dof motif, while for the endochitinase sequence differences did not modify the curvature profile. Individuals of the same cultivar carrying or not polymorphisms of both sequence and structure in a given motif have been isolated, and the expression of the corresponding genes is currently being analysed by means of real time PCR.

Promoter analysis of PR protein-coding genes in tomato (Lycopersicon esculentum Mill.) / P. Bettini; S. Sorace; M. Gori; M Buiatti. - STAMPA. - (2005), pp. 170-170. (Intervento presentato al convegno 2nd Solanaceae Genome Workshop 2005 tenutosi a Ischia (NA), Italy nel 25-29/09/2005).

Promoter analysis of PR protein-coding genes in tomato (Lycopersicon esculentum Mill.)

BETTINI, PRISCILLA PAOLA;GORI, MASSIMO;BUIATTI, MARCELLO
2005

Abstract

With the aim of analysing the presence of sequence and/or structural polymorphisms in regulatory regions and their relationship with gene expression, promoters of the PR protein-coding genes osmotin (GenBank accession AF093743), PR1a2 (Y08844) and endochitinase (A32906) were isolated in thirteen tomato cultivars and four wild species of the genus Lycopersicon (L. pennelli, L. minutum, L. pimpinellifolium, L. peruvianum). The sequences obtained have been aligned with the ClustalW algorithm (http://www.ebi.ac.uk/clustalw/), and conserved motifs regulating gene expression in the frame of the pathogen defense response have been detected using the PLACE (http://www.dna.affrc.go.jp/PLACE/) and PlantCARE (http://sphinx.rug.ac.be:8080/PlantCARE/) databases. Finally, an analysis of the curvature has been carried out at the bend it server (http://www3.icgeb.trieste.it/~dna/ bend_it.html). The results showed that for the osmotin and PR1a2 promoters nucleotide diversity is 15-fold higher between the Lycopersicon species than between the tomato cultivars, the difference being only 2-fold for endochitinase; the latter had also the lowest inter-specific and the highest intra-specific nucleotide diversity values. Correlations beween SNPs and structural polymorphisms were found for the osmotin and PR1a2 promoters in some regulatory boxes such as the W box, GT-1 consensus, Dof motif, while for the endochitinase sequence differences did not modify the curvature profile. Individuals of the same cultivar carrying or not polymorphisms of both sequence and structure in a given motif have been isolated, and the expression of the corresponding genes is currently being analysed by means of real time PCR.
2005
Proceedings 2nd Solanaceae Genome Workshop 2005
2nd Solanaceae Genome Workshop 2005
Ischia (NA), Italy
P. Bettini; S. Sorace; M. Gori; M Buiatti
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/655198
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