An immunoassay procedure for determination of progesterone in human plasma is described which utilizes chemiluminescence as the endpoint. The assay utilized progesterone-11-hemisuccinate-aminobutyl-ethyl-isoluminol (P-11-HS-ABEI) as the chemiluminescent marker conjugate and dextran coated charcoal for the separation of bound and free fractions of the ligand. An assay procedure for progesterone was established and validated in terms of sensitivity and precision, and assay results were compared with radioimmunoassay, using tritiated progesterone as the tracer. The two methods agreed well (n=35, r=0.96). The most important advantage of this assay is the elimination of problems inherent in the use of radioactive materials.
Luminescent immunoassay (LIA) for progesterone in a heterogeneous system / M. Pazzagli;J. B. Kim;G. Messeri;G. Martinazzo;F. Kohen;F. Franceschetti;A. Tommasi;R. Salerno;M. Serio. - In: CLINICA CHIMICA ACTA. - ISSN 0009-8981. - STAMPA. - 115:(1981), pp. 287-296. [10.1016/0009-8981(81)90242-4]
Luminescent immunoassay (LIA) for progesterone in a heterogeneous system.
PAZZAGLI, MARIO;SERIO, MARIO
1981
Abstract
An immunoassay procedure for determination of progesterone in human plasma is described which utilizes chemiluminescence as the endpoint. The assay utilized progesterone-11-hemisuccinate-aminobutyl-ethyl-isoluminol (P-11-HS-ABEI) as the chemiluminescent marker conjugate and dextran coated charcoal for the separation of bound and free fractions of the ligand. An assay procedure for progesterone was established and validated in terms of sensitivity and precision, and assay results were compared with radioimmunoassay, using tritiated progesterone as the tracer. The two methods agreed well (n=35, r=0.96). The most important advantage of this assay is the elimination of problems inherent in the use of radioactive materials.
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