The primary objective of this study consisted in: 1)formulation of methods for PCR amplification of transgenes, both promoters and terminators of transcription, and of junction zones between host DNA and vector DNA, in an experimental system used as a model made up of transgenic Nicotiana plants for the rat glucocorticoid receptor; 2)formulation of a series of protocols for detection of EPSPS transgene, resitant to glyphosate, or other sequences present in the expression box, which were introduced by the commercial version of Roundup Ready soy and in a series of derivatives, representing different stages of the production row.
Molecular methods for plant-to-product food row analysis / P.Bogani; A.Rosati; M.M.Spiriti; M.Buiatti. - STAMPA. - (2007), pp. 7-15. (Intervento presentato al convegno Workshop on "Innovative methods for monitoring and traceability of GMOs and food products containing GMOs" tenutosi a Roma, Italy nel 27 March).
Molecular methods for plant-to-product food row analysis
BOGANI, PATRIZIA;BUIATTI, MARCELLO
2007
Abstract
The primary objective of this study consisted in: 1)formulation of methods for PCR amplification of transgenes, both promoters and terminators of transcription, and of junction zones between host DNA and vector DNA, in an experimental system used as a model made up of transgenic Nicotiana plants for the rat glucocorticoid receptor; 2)formulation of a series of protocols for detection of EPSPS transgene, resitant to glyphosate, or other sequences present in the expression box, which were introduced by the commercial version of Roundup Ready soy and in a series of derivatives, representing different stages of the production row.
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