Objectives: The aim of the present study was to evaluate whether polymorphisms of the mannose-binding lectin (MBL-2) gene and MBL serum levels on admission to neonatal intensive care unit are associated with necrotizing enterocolitis (NEC) in preterm infants and to verify MBL expression in NEC bowels. Methods: In this retrospective cohort study, 107 neonates (41 with NEC and 66 controls) were included. MBL-2 genotyping for the promoter polymorphism 221 and for the exon 1 variant alleles at codons 52, 54, and 57 was performed. MBL levels were determined by enzymelinked immunosorbent assay in 55 infants. Immunohistochemical staining for MBL expression was performed on bowel specimens. The main study outcome was severe NEC (Bell stages II/III). Results: The 221 Y allele and the MBL-2 YY genotype were more frequent in neonates with severe NEC than in controls (P¼0.04 and P¼0.004, respectively). In the multivariate analysis, the MBL-2YA/YA genotype was associated with NEC (odds ratio¼3.03, 95% confidence interval 1.13%–8.13%, P¼0.024). Neonates with NEC had MBL level on admission >400 ng/mL more frequently than controls (P¼0.043). Among neonates with severe NEC, the deceased neonates were carriers of high or intermediate producing MBL-2 genotypes (P¼0.035). Finally, MBL was highly expressed in intestinal tissue from infants with NEC. Conclusions: MBL-2 genotypes associated with high MBL serum levels represent a risk factor for NEC. This finding, together with the MBL expression in bowel tissue, supports a role for MBL in the pathogenesis of NEC.

Association Between Mannose-bindingLectin Gene Polymorphisms and Necrotizing Enterocolitis in Preterm Infants / Prencipe G; Azzari C; Moriondo M; Devito R; Inglese R; Pezzullo M; Piersigilli F; Trucchi A; De Benedetti F; Auriti C.. - In: JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION. - ISSN 0277-2116. - STAMPA. - 55:(2012), pp. 160-165. [10.1097/MPG.0b013e31824e5f7a]

Association Between Mannose-bindingLectin Gene Polymorphisms and Necrotizing Enterocolitis in Preterm Infants.

AZZARI, CHIARA;MORIONDO, MARIA;
2012

Abstract

Objectives: The aim of the present study was to evaluate whether polymorphisms of the mannose-binding lectin (MBL-2) gene and MBL serum levels on admission to neonatal intensive care unit are associated with necrotizing enterocolitis (NEC) in preterm infants and to verify MBL expression in NEC bowels. Methods: In this retrospective cohort study, 107 neonates (41 with NEC and 66 controls) were included. MBL-2 genotyping for the promoter polymorphism 221 and for the exon 1 variant alleles at codons 52, 54, and 57 was performed. MBL levels were determined by enzymelinked immunosorbent assay in 55 infants. Immunohistochemical staining for MBL expression was performed on bowel specimens. The main study outcome was severe NEC (Bell stages II/III). Results: The 221 Y allele and the MBL-2 YY genotype were more frequent in neonates with severe NEC than in controls (P¼0.04 and P¼0.004, respectively). In the multivariate analysis, the MBL-2YA/YA genotype was associated with NEC (odds ratio¼3.03, 95% confidence interval 1.13%–8.13%, P¼0.024). Neonates with NEC had MBL level on admission >400 ng/mL more frequently than controls (P¼0.043). Among neonates with severe NEC, the deceased neonates were carriers of high or intermediate producing MBL-2 genotypes (P¼0.035). Finally, MBL was highly expressed in intestinal tissue from infants with NEC. Conclusions: MBL-2 genotypes associated with high MBL serum levels represent a risk factor for NEC. This finding, together with the MBL expression in bowel tissue, supports a role for MBL in the pathogenesis of NEC.
2012
55
160
165
Prencipe G; Azzari C; Moriondo M; Devito R; Inglese R; Pezzullo M; Piersigilli F; Trucchi A; De Benedetti F; Auriti C.
File in questo prodotto:
File Dimensione Formato  
2012 MBL prencipe.pdf

accesso aperto

Tipologia: Altro
Licenza: Open Access
Dimensione 176.51 kB
Formato Adobe PDF
176.51 kB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/676515
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 21
  • ???jsp.display-item.citation.isi??? 18
social impact