The construction of the plasmid pAF300, which contains the promoterless gene for chloramphenicol resistance fused to the broad host range conjugative vector pRK290, is described. Plasmid pAF300 could be used to identify transcription promoters by inserting DNA sequences in two cloning sites, HindIII and PstI. The vector efficiency was proved by cloning promoter sequences from Escherichia coli and Azospirillum brasilense which conferred chloramphenicol resistance to E. coli, A. brasilense and Pseudomonas putida.
A plasmid vector for the selection and the study of transcription promoters in Azospirillum brasilense / R. Fani; M. Bazzicalupo; F. Ricci; C. Schipani; M. Polsinelli. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - STAMPA. - 50:(1988), pp. 271-276.
A plasmid vector for the selection and the study of transcription promoters in Azospirillum brasilense
FANI, RENATO;BAZZICALUPO, MARCO;POLSINELLI, MARIO
1988
Abstract
The construction of the plasmid pAF300, which contains the promoterless gene for chloramphenicol resistance fused to the broad host range conjugative vector pRK290, is described. Plasmid pAF300 could be used to identify transcription promoters by inserting DNA sequences in two cloning sites, HindIII and PstI. The vector efficiency was proved by cloning promoter sequences from Escherichia coli and Azospirillum brasilense which conferred chloramphenicol resistance to E. coli, A. brasilense and Pseudomonas putida.
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