The application of gas chromatography-mass spectrometry (GC-MS) for the quantitative analysis of endogenous compounds as well as drugs in clinical biochemistry is taken into account. For this purpose, it is of the utmost importance the choice of a good internal standard (IS). It is demonstrated that the best IS in GC-MS is a stable isotope-labelled analogue of the analyte. In this case only we can speak of isotope dilution mass spectrometry. All the advantages and disadvantages inherent to the use of such a molecule as IS are discussed. The use of a calibration curve to perform the quantitative analysis is described, together with the criteria applied for the definition of the reliability of a method (precision, accuracy and limit of quantification). Examples taken from the literature are described.
Isotope dilution mass spectrometry: definitive methods and reference materials in clinical chemistry / Giovannini MG;Pieraccini G;Moneti G. - In: ANNALI DELL'ISTITUTO SUPERIORE DI SANITÀ. - ISSN 0021-2571. - STAMPA. - 27:(1991), pp. 401-410.
Isotope dilution mass spectrometry: definitive methods and reference materials in clinical chemistry.
GIOVANNINI, MARIA GRAZIA;PIERACCINI, GIUSEPPE;MONETI, GLORIANO
1991
Abstract
The application of gas chromatography-mass spectrometry (GC-MS) for the quantitative analysis of endogenous compounds as well as drugs in clinical biochemistry is taken into account. For this purpose, it is of the utmost importance the choice of a good internal standard (IS). It is demonstrated that the best IS in GC-MS is a stable isotope-labelled analogue of the analyte. In this case only we can speak of isotope dilution mass spectrometry. All the advantages and disadvantages inherent to the use of such a molecule as IS are discussed. The use of a calibration curve to perform the quantitative analysis is described, together with the criteria applied for the definition of the reliability of a method (precision, accuracy and limit of quantification). Examples taken from the literature are described.File | Dimensione | Formato | |
---|---|---|---|
1991 Ann Ist Super Sanità.pdf
Accesso chiuso
Tipologia:
Pdf editoriale (Version of record)
Licenza:
Tutti i diritti riservati
Dimensione
1.1 MB
Formato
Adobe PDF
|
1.1 MB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.