Cloning of several cDNA segments coding for human liver proteins.

EMBO J. 1983;2(1):57-61. Cloning of several cDNA segments coding for human liver proteins. Costanzo F, Castagnoli L, Dente L, Arcari P, Smith M, Costanzo P, Raugei G, Izzo P, Pietropaolo TC, Bougueleret L, Cimino F, Salvatore F, Cortese R. Source European Molecular Biology Laboratory, Heidelberg, FRG. Abstract A human cDNA library was constructed using M13 derivative vectors. The simple and rapid procedures for sequencing single-stranded DNA by the dideoxy chain termination method allowed a screening of individual clones directly by DNA sequence analysis. Some of these clones were identified as coding for: serum albumin, alpha1-antitrypsin, retinol-binding protein, prothrombin, haptoglobin, and metallothionein. Furthermore, a clone coding for aldolase B was tentatively identified on the basis of high sequence homology with rabbit muscle aldolase.