The mechanisms of nonclassical export of signal peptide-less proteins remain insufficiently understood. Here, we demonstrate that stress-induced unconventional export of FGF1, a potent and ubiquitously expressed mitogenic and proangiogenic protein, is associated with and dependent on the formation of membrane blebs and localized cell surface exposure of phosphatidylserine (PS). In addition, we found that the differentiation of promonocytic cells results in massive FGF1 release, which also correlates with membrane blebbing and exposure of PS. These findings indicate that the externalization of acidic phospholipids could be used as a pharmacological target to regulate the availability of FGF1 in the organism.

Phosphatidylserine externalization and membrane blebbing are involved in the nonclassical export of FGF1 / Kirov A;Al-Hashimi H;Solomon P;Mazur C;Thorpe PE;Sims PJ;Tarantini F;Kumar TK;Prudovsky I. - In: JOURNAL OF CELLULAR BIOCHEMISTRY. - ISSN 0730-2312. - STAMPA. - 113:(2012), pp. 956-966. [10.1002/jcb.23425]

Phosphatidylserine externalization and membrane blebbing are involved in the nonclassical export of FGF1.

TARANTINI, FRANCESCA;
2012

Abstract

The mechanisms of nonclassical export of signal peptide-less proteins remain insufficiently understood. Here, we demonstrate that stress-induced unconventional export of FGF1, a potent and ubiquitously expressed mitogenic and proangiogenic protein, is associated with and dependent on the formation of membrane blebs and localized cell surface exposure of phosphatidylserine (PS). In addition, we found that the differentiation of promonocytic cells results in massive FGF1 release, which also correlates with membrane blebbing and exposure of PS. These findings indicate that the externalization of acidic phospholipids could be used as a pharmacological target to regulate the availability of FGF1 in the organism.
2012
113
956
966
Kirov A;Al-Hashimi H;Solomon P;Mazur C;Thorpe PE;Sims PJ;Tarantini F;Kumar TK;Prudovsky I
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/697725
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