Background: Autologous fat transplantation (or lipofilling) is an excellent technique for correction of cosmetic defects. The success of the procedure relies strongly on the techniques of harvesting and transferring viable adipocytes. The purpose of this study was to evaluate effects of two harvesting methods and coenzyme Q(10) on the viability and apoptotic death of adipocytes collected for autologous lipofilling. Methods: Human adipose tissue from six patients was collected by Luer-Lok syringe according to Coleman's technique or by means of an aspirator with a 680-mmHg vacuum. Half of each sample collected using Coleman's technique was treated with 10 mu M Coenzyme Q(10), and the other half served as untreated control. Viability and apoptosis were assessed by immunoenzymatic, biochemical, and morphological methods. Results: The harvesting of adipose tissue by aspirator reduced the viability and increased apoptotic death significantly more than harvesting tissue using Coleman's technique. Biochemical and morphological analyses confirmed that treatment of adipose tissue with coenzyme Q, reduced and even inhibited apoptotic death of harvested adipocytes. Conclusion: Coenzyme Q(10) can rescue adipocytes from stress-induced apoptotic death.

Autologous lipofilling: Coenzyme Q(10) can rescue adipocytes from stress-induced apoptotic death / Witort EJ; Pattarino J; Papucci L; Schiavone N; Donnini M; Lapucci A; Lulli M; Lo Russo G; Mori A; Dini M; Capaccioli S. - In: PLASTIC AND RECONSTRUCTIVE SURGERY. - ISSN 0032-1052. - STAMPA. - 119:(2007), pp. 1191-1199. (Intervento presentato al convegno 9th International Congress of Italian and American Plastic Surgeons tenutosi a New Orleans, LA. nel SEP 20, 2004) [10.1097/01.prs.0000258395.81926.d8].

Autologous lipofilling: Coenzyme Q(10) can rescue adipocytes from stress-induced apoptotic death.

PATTARINO, JACOPO;PAPUCCI, LAURA;SCHIAVONE, NICOLA;DONNINI, MARTINO;LAPUCCI, ANDREA;LULLI, MATTEO;LO RUSSO, GIULIA;MORI, ANDREA;DINI, MARIO;CAPACCIOLI, SERGIO
2007

Abstract

Background: Autologous fat transplantation (or lipofilling) is an excellent technique for correction of cosmetic defects. The success of the procedure relies strongly on the techniques of harvesting and transferring viable adipocytes. The purpose of this study was to evaluate effects of two harvesting methods and coenzyme Q(10) on the viability and apoptotic death of adipocytes collected for autologous lipofilling. Methods: Human adipose tissue from six patients was collected by Luer-Lok syringe according to Coleman's technique or by means of an aspirator with a 680-mmHg vacuum. Half of each sample collected using Coleman's technique was treated with 10 mu M Coenzyme Q(10), and the other half served as untreated control. Viability and apoptosis were assessed by immunoenzymatic, biochemical, and morphological methods. Results: The harvesting of adipose tissue by aspirator reduced the viability and increased apoptotic death significantly more than harvesting tissue using Coleman's technique. Biochemical and morphological analyses confirmed that treatment of adipose tissue with coenzyme Q, reduced and even inhibited apoptotic death of harvested adipocytes. Conclusion: Coenzyme Q(10) can rescue adipocytes from stress-induced apoptotic death.
2007
9th International Congress of Italian and American Plastic Surgeons
New Orleans, LA.
SEP 20, 2004
Witort EJ; Pattarino J; Papucci L; Schiavone N; Donnini M; Lapucci A; Lulli M; Lo Russo G; Mori A; Dini M; Capaccioli S
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/721935
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