We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other sample manipulation. The drawback of SedNMR is that samples are intrinsically diluted and this is detrimental for the signal intensity. Ultracentrifugal devices can be used to increase the amount of sample inside the rotor, overcoming the intrinsic sensitivity limitation of the method. We designed two different devices and we here report the directions for using such devices and the relevant equations for determining the parameters for sedimentation.
On the use of ultracentrifugal devices for sedimented solute NMR / Ivano Bertini; Frank Engelke; Leonardo Gonnelli; Benno Knott; Claudio Luchinat; David Osen; Enrico Ravera. - In: JOURNAL OF BIOMOLECULAR NMR. - ISSN 0925-2738. - STAMPA. - 54:(2012), pp. 123-127. [10.1007/s10858-012-9657-y]
On the use of ultracentrifugal devices for sedimented solute NMR
BERTINI, IVANO;GONNELLI, LEONARDO;LUCHINAT, CLAUDIO;RAVERA, ENRICO
2012
Abstract
We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other sample manipulation. The drawback of SedNMR is that samples are intrinsically diluted and this is detrimental for the signal intensity. Ultracentrifugal devices can be used to increase the amount of sample inside the rotor, overcoming the intrinsic sensitivity limitation of the method. We designed two different devices and we here report the directions for using such devices and the relevant equations for determining the parameters for sedimentation.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.