Myocardial regeneration with stem cell (SC) transplantation is an emerging therapeutic strategy to improve cardiac function after infarction. SC are pluripotent cells that can proliferate, self-renew, and differentiate into cardiomyocytes. A fundamental question is how SC acquire a cardiac functional phenotype. To address this point we used mouse embryonic SC (mES) induced to differentiate via embryo-like aggregates. In this model we identified cardiac functional markers by studying the cellular electrophysiological properties. Results show that differentiation of mES gives rise to cell aggregates featuring spontaneous contractile activity. Patch-clamp recordings show that their cellular excitability (action potential and ionic currents) closely resembles that of native cardiomyocytes. Thus, mES derived cardiomyocytes may be a useful model for investigating cardiac development and key factors involved in the control of cardiac differentiation.

Cardiac myocytes functional markers in embryonic stem cell-derived myocytes / L. Sartiani; S. Brogioni; F. Stillitano; M. Jaconi; R Squecco ; E. Cerbai; A. Mugelli. - In: JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY. - ISSN 0142-4319. - STAMPA. - 25:(2004), pp. 281-281.

Cardiac myocytes functional markers in embryonic stem cell-derived myocytes

SARTIANI, LAURA;STILLITANO, FRANCESCA;SQUECCO, ROBERTA;CERBAI, ELISABETTA;MUGELLI, ALESSANDRO
2004

Abstract

Myocardial regeneration with stem cell (SC) transplantation is an emerging therapeutic strategy to improve cardiac function after infarction. SC are pluripotent cells that can proliferate, self-renew, and differentiate into cardiomyocytes. A fundamental question is how SC acquire a cardiac functional phenotype. To address this point we used mouse embryonic SC (mES) induced to differentiate via embryo-like aggregates. In this model we identified cardiac functional markers by studying the cellular electrophysiological properties. Results show that differentiation of mES gives rise to cell aggregates featuring spontaneous contractile activity. Patch-clamp recordings show that their cellular excitability (action potential and ionic currents) closely resembles that of native cardiomyocytes. Thus, mES derived cardiomyocytes may be a useful model for investigating cardiac development and key factors involved in the control of cardiac differentiation.
2004
L. Sartiani; S. Brogioni; F. Stillitano; M. Jaconi; R Squecco ; E. Cerbai; A. Mugelli
File in questo prodotto:
File Dimensione Formato  
Sartiani_J Muscle Res C M_2004.pdf

Accesso chiuso

Tipologia: Altro
Licenza: Tutti i diritti riservati
Dimensione 9.72 kB
Formato Adobe PDF
9.72 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/771947
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact