Surface Plasmon Resonance imaging (SPRi) is an advanced optical transducer for the DNA affinity biosensors. SPRi signal enhancement of a DNA biosensor is a goal in developing innovative devices, especially for clinical applications. Nanoparticles (NPs) play a forefront role in signal enhancement in biosensor-based analysis, using different transduction principles. This work focused on the use of NPs for gold chip surface nanostructuring with the aim to study influence of this modification on DNA-based sensing using SPRi transduction. In particular gold NPs of different size and materials were immobilized through dithiol layer on gold biochip surface and further functionalized with thiolated DNA probe. Plasmon curves were studied for assessing the presence of the nanostructure and the influence of the nanostructure in SPRi signal was evaluated for each structure in hybridization with complementary oligonucleotide.

Coupling nanotechnology to optical affinity sensing: the case of surface plasmon resonance imaging for DNA detection / M.L. Ermini; S. Mariani; F. Bellissima; S. Scarano; M. Bonini; M. Minunni. - ELETTRONICO. - 162:(2014), pp. 103-106. ((Intervento presentato al convegno CONVEGNO NAZIONALE SENSORI Innovazione, attualità e prospettive tenutosi a ROMA nel 15-17 FEBBRAIO 2012 [10.1007/978-1-4614-3860-1_17].

Coupling nanotechnology to optical affinity sensing: the case of surface plasmon resonance imaging for DNA detection

ERMINI, MARIA LAURA;MARIANI, STEFANO;BELLISSIMA, FRANCESCA;SCARANO, SIMONA;BONINI, MASSIMO;MINUNNI, MARIA
2014

Abstract

Surface Plasmon Resonance imaging (SPRi) is an advanced optical transducer for the DNA affinity biosensors. SPRi signal enhancement of a DNA biosensor is a goal in developing innovative devices, especially for clinical applications. Nanoparticles (NPs) play a forefront role in signal enhancement in biosensor-based analysis, using different transduction principles. This work focused on the use of NPs for gold chip surface nanostructuring with the aim to study influence of this modification on DNA-based sensing using SPRi transduction. In particular gold NPs of different size and materials were immobilized through dithiol layer on gold biochip surface and further functionalized with thiolated DNA probe. Plasmon curves were studied for assessing the presence of the nanostructure and the influence of the nanostructure in SPRi signal was evaluated for each structure in hybridization with complementary oligonucleotide.
Lecture Notes in Electrical Engineering
CONVEGNO NAZIONALE SENSORI Innovazione, attualità e prospettive
ROMA
15-17 FEBBRAIO 2012
M.L. Ermini; S. Mariani; F. Bellissima; S. Scarano; M. Bonini; M. Minunni
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/776853
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