Abstract: To investigate the time sequence of cardiac growth factor formation, echocardiographic and hemodynamic measurements were performed at scheduled times, and mRNAs for angiotensinogen, prepro-endothelin-1 (ppET-1), and insulin-like growth factor I (IGF-I) were quantified with RT-PCR and localized with in situ hybridization in pigs (fluothane anesthesia) by use of pressure or volume overload (aortic banding and aorta-cava fistula, respectively). Relative peptide formation was also measured by radioimmunoassay. In pressure overload, angiotensinogen and ppET-1 mRNA overexpression on myocytes (13 times vs. sham at 3 h and 112 times at 6 h, respectively) was followed by recovery (12 h) of initially decreased (0.5-6 h) myocardial contractility. In volume overload, contractility was not decreased, the angiotensinogen gene was slightly upregulated at 6 h (6.7 times), and ppET-1 was not overexpressed. IGF-I mRNA was overexpressed on myocytes (at 24 h) in both volume and pressure overload (14 times and 37 times, respectively). In the latter setting, a second ppET-1 overexpression was detectable on myocytes at 7 days. In conclusion, acute cardiac adaptation responses involve different growth factor activation over time in pressure versus volume overload; growth factors initially support myocardial contractility and thereafter induce myocardial hypertrophy.

Early sequence of cardiac adaptations and growth factor formation in pressure- and volume-overload hypertrophy / P.A. MODESTI; S. VANNI; I. BERTOLOZZI; I. CECIONI; G. POLIDORI; R. PANICCIA; B. BANDINELLI; A. PERNA; P. LIGUORI; M. BODDI; G. GALANTI; G.G. NERI SERNERI. - In: AMERICAN JOURNAL OF PHYSIOLOGY. HEART AND CIRCULATORY PHYSIOLOGY. - ISSN 0363-6135. - STAMPA. - 279:(2000), pp. H976-H985.

Early sequence of cardiac adaptations and growth factor formation in pressure- and volume-overload hypertrophy

MODESTI, PIETRO AMEDEO
Writing – Review & Editing
;
VANNI, SIMONE;BERTOLOZZI, IACOPO;CECIONI, ILARIA;PANICCIA, RITA;BANDINELLI, BRUNELLA;BODDI, MARIA;GALANTI, GIORGIO;NERI SERNERI, GIAN GASTONE
2000

Abstract

Abstract: To investigate the time sequence of cardiac growth factor formation, echocardiographic and hemodynamic measurements were performed at scheduled times, and mRNAs for angiotensinogen, prepro-endothelin-1 (ppET-1), and insulin-like growth factor I (IGF-I) were quantified with RT-PCR and localized with in situ hybridization in pigs (fluothane anesthesia) by use of pressure or volume overload (aortic banding and aorta-cava fistula, respectively). Relative peptide formation was also measured by radioimmunoassay. In pressure overload, angiotensinogen and ppET-1 mRNA overexpression on myocytes (13 times vs. sham at 3 h and 112 times at 6 h, respectively) was followed by recovery (12 h) of initially decreased (0.5-6 h) myocardial contractility. In volume overload, contractility was not decreased, the angiotensinogen gene was slightly upregulated at 6 h (6.7 times), and ppET-1 was not overexpressed. IGF-I mRNA was overexpressed on myocytes (at 24 h) in both volume and pressure overload (14 times and 37 times, respectively). In the latter setting, a second ppET-1 overexpression was detectable on myocytes at 7 days. In conclusion, acute cardiac adaptation responses involve different growth factor activation over time in pressure versus volume overload; growth factors initially support myocardial contractility and thereafter induce myocardial hypertrophy.
2000
279
H976
H985
P.A. MODESTI; S. VANNI; I. BERTOLOZZI; I. CECIONI; G. POLIDORI; R. PANICCIA; B. BANDINELLI; A. PERNA; P. LIGUORI; M. BODDI; G. GALANTI; G.G. NERI SE...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/777162
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