Haematic cells, excited with radiation of suitable wavelength, give rise to a natural fluorescence (NF) emission. This paper investigates NF to develop new techniques for applications in both basic research and medical diagnostics. Results show that the cell populations examined exhibit peculiar emission bands. The intracellular fluorescence pattern reveals that fluoresence is mainly located at cytoplasmic-level, thus related to the metabolic processes of the cells. The photophysical properties of cells appear different among the normal populations and between normal and leukaemic ones. Therefore the recognition of the various cellular elements, according to their fluorescence emission, is possible.
Image analysis of cell natural fluorescence. Diagnostic applications in haematology / M. Monici;G. Agati;P. Mazzinghi;F. Fusi;P. A. Bernabei;I. Landini;P. R. Ferrini;R. Pratesi. - STAMPA. - 2928:(1996), pp. 180-187. (Intervento presentato al convegno Biomedical Systems and Technologies tenutosi a Vienna nel 1996) [10.1117/12.259969].
Image analysis of cell natural fluorescence. Diagnostic applications in haematology.
FUSI, FRANCO;PRATESI, RICCARDO
1996
Abstract
Haematic cells, excited with radiation of suitable wavelength, give rise to a natural fluorescence (NF) emission. This paper investigates NF to develop new techniques for applications in both basic research and medical diagnostics. Results show that the cell populations examined exhibit peculiar emission bands. The intracellular fluorescence pattern reveals that fluoresence is mainly located at cytoplasmic-level, thus related to the metabolic processes of the cells. The photophysical properties of cells appear different among the normal populations and between normal and leukaemic ones. Therefore the recognition of the various cellular elements, according to their fluorescence emission, is possible.
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