Retrotransposable elements are common parts of plants genomes (Heslop-Harrison et al ., 1997) showing high variability (Le et al. , 2000) and complex taxonomy; their high stress-related mobility in the genome contribute to genome evolution (Wendel & Wessler, 2000) and the insertion in coding regions imply demonstrated regulative action (White et al., 1994; Liu & Wendel, 2000). The presence of retrotransposons is widely reported for herbaceous and agronomic species (Wang et al ., 1997; Rogers & Pauls, 2000) whilst in forest trees only for conifers (Kamm et al ., 1996; L’Homme et al ., 2000). During a genetic diversity screening in beech’s (Fagus sylvatica L.) population of southern Italy using RAPD markers, we argued a peculiar role for two high frequency markers (freq. > 98% among the 700 individuals analysed). Southern Blotting analysis demonstrated markers uniqueness and the effective presence of fragments in the samples. Sequencing and successive BLAST alignments scored high similarity to Arabidopsis ’ and Zea mais ’ retrotransposons. The retrotransposons discovered (GeneBank accession n. AF405557, AF405555) in Beech belong to the groups of Copia-like and non-LTR retrotransposable elements. The potential regulative function of these two elements can be used as functional markers useful in genetic improvement strategies. The work is going ahead in screening other broadleaf for retrotransposable elements to use in adaptation studies and phylogenetic analysis.
LTR and non-LTR retrotransposons are common genetic components in Fagus sylvatica L. genome / Emiliani G.; Paffetti D.; Giannini R.. - ELETTRONICO. - (2002), pp. S4e--. (Intervento presentato al convegno XLVI SIGA ANNUAL CONGRESS tenutosi a Giardini Naxos (ME) nel September 18/21, 2002).
LTR and non-LTR retrotransposons are common genetic components in Fagus sylvatica L. genome
EMILIANI, GIOVANNI;PAFFETTI, DONATELLA;GIANNINI, RAFFAELLO
2002
Abstract
Retrotransposable elements are common parts of plants genomes (Heslop-Harrison et al ., 1997) showing high variability (Le et al. , 2000) and complex taxonomy; their high stress-related mobility in the genome contribute to genome evolution (Wendel & Wessler, 2000) and the insertion in coding regions imply demonstrated regulative action (White et al., 1994; Liu & Wendel, 2000). The presence of retrotransposons is widely reported for herbaceous and agronomic species (Wang et al ., 1997; Rogers & Pauls, 2000) whilst in forest trees only for conifers (Kamm et al ., 1996; L’Homme et al ., 2000). During a genetic diversity screening in beech’s (Fagus sylvatica L.) population of southern Italy using RAPD markers, we argued a peculiar role for two high frequency markers (freq. > 98% among the 700 individuals analysed). Southern Blotting analysis demonstrated markers uniqueness and the effective presence of fragments in the samples. Sequencing and successive BLAST alignments scored high similarity to Arabidopsis ’ and Zea mais ’ retrotransposons. The retrotransposons discovered (GeneBank accession n. AF405557, AF405555) in Beech belong to the groups of Copia-like and non-LTR retrotransposable elements. The potential regulative function of these two elements can be used as functional markers useful in genetic improvement strategies. The work is going ahead in screening other broadleaf for retrotransposable elements to use in adaptation studies and phylogenetic analysis.File | Dimensione | Formato | |
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